Serum IL-21 levels associated with chronic hepatitis B and hepatitis B-related liver failure.
Serum IL-21 levels associated with chronic hepatitis B and hepatitis B-related liver failure.
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DOI：
10.3892/etm.2014.1533
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发表时间：
2014-04
影响因子：
2.7
通讯作者：
Zhao YR
中科院分区：
文献类型：
--
作者：
Chen HM;Liu HL;Yang YC;Cheng XL;Wang YF;Xing FF;Zhao YR
本研究旨在探讨白细胞介素（IL）-21在慢性B型肝炎病毒（HBV）感染中的作用。IL-21刺激T和B细胞应答，并在控制慢性病毒感染中发挥作用。采用酶免疫法检测了109例慢性HBV感染者血清IL-21水平，并与19例健康对照者进行了比较。通过细胞内细胞因子染色和流式细胞术评估外周血中产生IL-21的T细胞的比例。慢性B型肝炎（CH B）患者血清IL-21水平为303.54±152.77 pg/ml，HC患者血清IL-21水平为68.24±9.06 pg/ml（P=0.003）。乙型肝炎慢加急性肝衰竭（HB-ACLF）患者血清IL-21水平为455.38±412.38 pg/ml，与HC组比较差异有统计学意义（P=0.000）。HB-ACLF患者血清IL-21水平最高（455.38±412.38 pg/ml），与CHB患者比较差异有显著性（P=0.04）。肝硬化患者血清IL-21水平也升高，但与HC相比无统计学差异（P=0.82）。IL-21+ CD 4+细胞的频率也比HC增加，并与外周血中淋巴细胞的数量和百分比相关。CHB和HB-ACLF患者血清IL-21水平升高。慢性乙型肝炎患者血清IL-21水平相对较低可能与HBV感染的持续性有关。HB-ACLF中较高的血清水平可能激活T和B细胞以消除病毒或通过释放炎性细胞因子损伤肝脏。
The aim of the present study was to investigate the role of interleukin (IL)-21 in chronic hepatitis B virus (HBV) infection. IL-21 stimulates T and B cell responses and plays a role in the control of chronic viral infections. Serum IL-21 levels were measured by enzyme immunoassay in 109 patients with chronic HBV infection at various clinical stages, as well as in 19 healthy controls (HCs). The proportion of T cells producing IL-21 in the peripheral blood was assessed by intracellular cytokine staining and flow cytometry. Mean serum IL-21 levels in patients with chronic hepatitis B (CHB) and the HCs were 303.54±152.77 pg/ml and 68.24±9.06 pg/ml, respectively (P=0.003). In addition, the mean serum IL-21 level in patients with hepatitis B-related acute-on-chronic liver failure (HB-ACLF) was 455.38±412.38 pg/ml, which exhibited a statistically significant difference when compared with the HCs (P=0.000). Serum IL-21 levels were highest in the patients with HB-ACLF (455.38±412.38 pg/ml) and exhibited a significant difference when compared with the CHB patients (P=0.04). The mean serum IL-21 levels in patients with cirrhosis also increased, but there was no statistically significant difference when compared with the HCs (P=0.82). The frequency of IL-21+CD4+ cells also increased compared with the HCs and correlated with the number and percentage of lymphocytes in the peripheral blood. Serum IL-21 levels increased in CHB and HB-ACLF patients. Relatively low serum IL-21 levels in CHB may have a causal role in the persistence of HBV infection. Higher serum levels in HB-ACLF may activate T and B cells to eliminate the virus or injure the liver via the release of inflammatory cytokines.
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影响因子：
6.1
作者：
Cho, SH;Stanciu, LA;Johnston, SL
通讯作者：
Johnston, SL
影响因子：
4.4
作者：
Iannello, Alexandre;Boulassel, Mohamed-Rachid;Ahmad, Ali
通讯作者：
Ahmad, Ali
DOI：
10.1073/pnas.0807309106
发表时间：
2009-02-03
影响因子：
11.1
作者：
Bubier, Jason A.;Sproule, Thomas J.;Roopenian, Derry C.
通讯作者：
Roopenian, Derry C.
影响因子：
8.8
作者：
Lavanchy, D.
通讯作者：
Lavanchy, D.
影响因子：
3.3
作者：
Green, KJ;Rowbottom, DG;Mackinnon, LT
通讯作者：
Mackinnon, LT