Potential subunit vaccine against Mycoplasma pulmonis purified by a protective monoclonal antibody.
Potential subunit vaccine against Mycoplasma pulmonis purified by a protective monoclonal antibody.
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通过保护性单克隆抗体纯化的针对肺支原体的潜在亚单位疫苗。
DOI：
10.1016/0264-410x(91)90150-5
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发表时间：
1991
期刊：
影响因子：
5.5
通讯作者：
Murphree,SS
中科院分区：
文献类型：
--
作者：
Lai,WC;Bennett,M;Pakes,SP;Murphree,SS
制备了抗肺支原体（MP）的单克隆抗体（mAb），其中一些在治疗BALB/c小鼠实验性感染中具有高度保护性。单克隆抗体抑制MP在体外的生长，并阻止MP附着到成纤维细胞或红细胞。三种单独的mAb识别54-76%的MP临床分离株，三种mAb一起检测到所有54种分离株。我们用单克隆抗体通过亲和柱层析纯化抗原。纯化的抗原用于接种小鼠和免疫兔产生的抗体能够显着抑制血清和气管灌洗液中的生长。用不同剂量的MP高毒力T2株攻击接种疫苗的小鼠。对感染小鼠肺中活MP微生物和组织病理学变化的测定表明，如果攻毒剂量为103-105，而不是107，c.f.u.，则小鼠受到保护。免疫兔血清用于被动转移免疫小鼠。血清对1 × 106c.f.u. T2 MP。我们的结论是，MP抗原纯化，该协议可以提供一个安全的疫苗，至少在小鼠中对抗这种疾病。
Monoclonal antibodies (mAbs) were produced againstMycoplasma pulmonis(MP); some were highly protective in the treatment of experimental infections of BALB/c mice. The mAbs inhibited MP growthin vitroand prevented the attachment of MP to fibroblasts or to red blood cells. Three separate mAbs recognized 54–76% of 54 clinical isolates of MP, and the three together detected all 54 isolates. We used the mAbs to purify the antigens by affinity column chromatography. The purified antigen used to vaccinate mice and to immunize rabbits produced antibodies capable of significant growth inhibition in sera and tracheolung lavage fluids. The vaccinated mice were challenged with various doses of a highly virulent T2 strain of MP. Assays for viable MP organisms and for histopathological changes in the lungs of infected mice indicated that mice were protected if the challenge dose was 103–105, but not 107, c.f.u. The sera of immunized rabbits were used to passively transfer immunity to mice. The sera provided complete protection against 1 × 106c.f.u. T2 MP. We conclude that MP antigens purified by this protocol can provide a safe vaccine against this disease, at least in mice.
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使用生长抑制单克隆抗体分化引起传染性山羊胸膜肺炎的 F38 支原体
影响因子：
3.1
作者：
F. R. Rurangirwa;Travis C. McGuire;Anthony J. Musoke;A. Kibor
通讯作者：
A. Kibor
DOI：
10.1111/j.1749-6632.1973.tb45665.x
发表时间：
1973-01-01
期刊：
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES-SERIES
影响因子：
--
作者：
CASSELL, GH;LINDSEY, JR;BAKER, HJ
通讯作者：
BAKER, HJ
硫酸葡聚糖增强小鼠对非活肺支原体的迟发性超敏反应
影响因子：
3.1
作者：
M. Kishima;C. Kuniyasu;M. Nakagawa
通讯作者：
M. Nakagawa
在 Aotus 猴中诱导针对单克隆抗体定义的恶性疟原虫抗原的保护性免疫需要强佐剂
影响因子：
3.1
作者：
W. A. Siddiqui;L. Tam;S. Kan;K. Kramer;S. Case;K. Palmer;K. Yamaga;G. Hui
通讯作者：
G. Hui
影响因子：
3
作者：
DENNY, FW;ALLISON, AC;TAYLORRO.D
通讯作者：
TAYLORRO.D