The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha-catenin with vascular endothelial cadherin (VE-cadherin).
The molecular organization of endothelial cell to cell junctions: differential association of plakoglobin, beta-catenin, and alpha-catenin with vascular endothelial cadherin (VE-cadherin).
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DOI：
10.1083/jcb.129.1.203
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发表时间：
1995-04
影响因子：
7.8
通讯作者：
DEJANA, E
中科院分区：
文献类型：
--
作者：
LAMPUGNANI, MG;CORADA, M;CAVEDA, L;BREVIARIO, F;AYALON, O;GEIGER, B;DEJANA, E
在本文中，我们报道了含有细胞类型特异性血管内皮细胞钙粘附素(VE-cadherin或cadherin-5)的内皮间连接的组装是一个动态过程，受细胞功能状态的影响。免疫荧光双标记法显示，VE-钙粘附素、α-连环素和β-连环素共存于稀疏和融合的内皮细胞单层的细胞间接触区。相反，只有在紧密融合的细胞中，伴随着蛋白和信使核糖核酸水平的增加，白蛋白才与细胞间的连接相关。此外，在紧密堆积的单层中，与VE-钙粘附素共沉淀的白蛋白数量增加。融合EC单层的人工损伤导致VE-钙粘蛋白、α-连环蛋白、β-连环蛋白和白蛋白的重大重组。所有这些蛋白在迁移到病变中的EC边界上的强度都降低。相反，位于迁移前锋正后方的EC保留了连接的VE-钙粘附素、α-连环素和β-连环素，而这些部位没有蛋白。与此观察一致的是，在迁移性EC中，与VE-钙粘附素共沉淀的白蛋白数量减少。这些数据表明，VE-钙粘附素、α-连环素和β-连环素在细胞间黏附的早期阶段就已经相互关联，并在新生细胞接触时变得容易组织。另一方面，只有当细胞接近汇合点时，蛋白才会与连接点结合。当细胞迁移时，这个顺序是颠倒的，即首先解离蛋白，然后VE-钙粘蛋白、α-连环蛋白和β-连环蛋白从连接处解离。蛋白与连接的晚期结合表明，虽然VE-钙粘蛋白/α-连环蛋白/β-连环蛋白复合体可以作为EC之间的早期识别机制，但成熟的、细胞骨架结合的连接的形成需要蛋白的合成和组织。
In this paper we report that the assembly of interendothelial junctions containing the cell type-specific vascular endothelial cadherin (VE- cadherin or cadherin-5) is a dynamic process which is affected by the functional state of the cells. Immunofluorescence double labeling of endothelial cells (EC) cultures indicated that VE-cadherin, alpha- catenin, and beta-catenin colocalized in areas of cell to cell contact both in sparse and confluent EC monolayers. In contrast, plakoglobin became associated with cell-cell junctions only in tightly confluent cells concomitantly with an increase in its protein and mRNA levels. Furthermore, the amount of plakoglobin coimmunoprecipitated with VE- cadherin, increased in closely packed monolayers. Artificial wounding of confluent EC monolayers resulted in a major reorganization of VE- cadherin, alpha-catenin, beta-catenin, and plakoglobin. All these proteins decreased in intensity at the boundaries of EC migrating into the lesion. In contrast, EC located immediately behind the migrating front retained junctional VE-cadherin, alpha-catenin, and beta-catenin while plakoglobin was absent from these sites. In line with this observation, the amount of plakoglobin coimmunoprecipitated with VE- cadherin decreased in migrating EC. These data suggest that VE- cadherin, alpha-catenin, and beta-catenin are already associated with each other at early stages of intercellular adhesion and become readily organized at nascant cell contacts. Plakoglobin, on the other hand, associates with junctions only when cells approach confluence. When cells migrate, this order is reversed, namely, plakoglobin dissociates first and, then, VE-cadherin, alpha-catenin, and beta-catenin disassemble from the junctions. The late association of plakoglobin with junctions suggests that while VE-cadherin/alpha-catenin/beta- catenin complex can function as an early recognition mechanism between EC, the formation of mature, cytoskeleton-bound junctions requires plakoglobin synthesis and organization.
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DOI：
10.1083/jcb.118.3.671
发表时间：
1992-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Knudsen KA;Wheelock MJ
通讯作者：
Wheelock MJ
影响因子：
7.8
作者：
Hinck, L;Nelson, W J;Papkoff, J
通讯作者：
Papkoff, J
DOI：
10.1083/jcb.123.6.1857
发表时间：
1993-12
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bradley RS;Cowin P;Brown AM
通讯作者：
Brown AM
蛋白激酶抑制剂可预防MDCK上皮细胞中低细胞外钙诱导的连接解离。
影响因子：
7.8
作者：
Citi, S
通讯作者：
Citi, S
影响因子：
64.5
作者：
HIRANO, S;KIMOTO, N;TAKEICHI, M
通讯作者：
TAKEICHI, M