WTX inhibits gastric cancer migration through the reversal of epithelial-mesenchymal transition.
WTX inhibits gastric cancer migration through the reversal of epithelial-mesenchymal transition.
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DOI：
10.3892/ol.2018.9309
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发表时间：
2018-10
期刊：
影响因子：
2.9
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Ye D;Ma W;Xu J;Zhu G;Liu D;Liu C;Ding Y;Zhang Q
本研究旨在探讨Wilms肿瘤基因在X染色体(WTX)上的表达是否影响胃癌细胞上皮-间充质转化(EMT)过程和迁移。建立稳定表达WTX的AGS细胞(AGS.W)，并用流式细胞仪进行分析。荧光显微镜、逆转录-定量聚合酶链式反应和Western blotting证实了过表达的有效性。采用免疫印迹和免疫荧光方法分析EMT相关蛋白的表达。用Transwell伤口愈合试验检测细胞的迁移能力。与对照细胞(AGS.veh)相比，AGS.w胃癌细胞WTX在mRNA(P<；0.05)和蛋白水平(P<；0.05)的表达均显著增加。形态观察表明，AGS.w细胞转变为纺锤形，而AGS.veh细胞保持圆形或椭圆形。此外，Western blotting和免疫荧光实验证实，与AGS.veh细胞相比，AGS.W细胞上皮标志物上皮钙粘附素的表达水平显著增加，而间充质标志物神经钙粘附素、β-连环蛋白和波形蛋白的表达水平显著降低。此外，与AGS.veh细胞相比，WTX过表达降低了AGS.w细胞的迁移能力。外源表达WTX通过逆转EMT抑制胃癌细胞迁移。本研究的结果描述了一个分子特征，该特征可能是未来胃癌治疗策略的一个有希望的靶点。
The aim of the present study was to investigate whether the expression of Wilms' tumor gene on X chromosome (WTX) affected the epithelial-mesenchymal transition (EMT) process and migration of gastric cancer cells. Stable WTX-overexpressing AGS cells (AGS.W) were established and analyzed by flow cytometry. The efficiency of the overexpression was verified by fluorescence microscopy, reverse transcription-quantitative polymerase chain reaction and western blotting. To analyze the expression of EMT-associated proteins, western blotting and immunofluorescence assays were performed. The migratory capability of the cells was detected by Transwell wound-healing assays, respectively. Compared with that of the control cells (AGS.veh), WTX expression was notably increased at mRNA (P<0.05) and protein levels (P<0.05) in the AGS.W gastric cancer cells. Morphological observations indicated that AGS.W cells transformed into spindle shapes, compared to AGS.veh cells, which maintained round or oval shapes. Furthermore, western blotting and immunofluorescence validated that the expression level of the epithelial marker epithelial-cadherin was significantly increased, whereas the expression levels of the mesenchymal markers neural-cadherin, β-catenin and vimentin were significantly decreased in the AGS.W cells compared with those in the AGS.veh cells. In addition, the overexpression of WTX decreased the migratory ability of AGS.W cells compared with AGS.veh cells. Exogenous expression of WTX inhibited gastric cancer cell migration by reversing EMT. The results of the present study describe a molecular feature that may be a promising target for future gastric cancer therapy strategies.
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影响因子：
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作者：
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通讯作者：
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影响因子：
56.9
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
5.2
作者：
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通讯作者：
Blons H