Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new members.
Origin and evolution of the archaeo-eukaryotic primase superfamily and related palm-domain proteins: structural insights and new members.
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考古 - 核核原始酶超家族和相关棕榈域蛋白的起源和进化：结构见解和新成员。
DOI：
10.1093/nar/gki702
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发表时间：
2005
影响因子：
14.9
通讯作者：
Aravind, L
中科院分区：
文献类型：
--
作者：
Iyer, LM;Koonin, EV;Leipe, DD;Aravind, L
我们报告了一个深入的计算研究的蛋白质序列和结构的超家族的古真核生物引物（AEPs）。这一分析极大地扩展了aep的多样性范围，揭示了该超家族所有成员共有的独特活性位点。特别是，研究表明真核生物核胞质大DNA病毒，包括痘病毒、阿斯法病毒、虹膜病毒、藻病毒和mimivirus，编码一个不同家族的AEPs，其中还包括疱疹病毒引物，其与AEPs的关系以前尚未被认识到。许多真核生物基因组，包括脊索动物和植物，编码了这些预测病毒引物的先前未被表征的同源物，这可能涉及新的DNA修复途径。在更深层次的进化联系中，结构比较表明，AEPs，参与质粒和病毒中滚动圈复制起始的核酸酶，以及乳头状瘤和多瘤病毒的起始结合域，是从一个共同的祖先蛋白质进化而来的，该蛋白质可能参与了DNA复制起始的蛋白质启动机制。对原核生物和病毒的多结构域蛋白结构和基因邻域的上下文分析揭示了AEPs与不相关的dna型引物之间的显著相似之处，特别是与相同的解旋酶库密切相关。这些观察结果表明，这两类引物在功能上是对等的，它们似乎在各种染色体外复制子中反复相互取代。
We report an in-depth computational study of the protein sequences and structures of the superfamily of archaeo-eukaryotic primases (AEPs). This analysis greatly expands the range of diversity of the AEPs and reveals the unique active site shared by all members of this superfamily. In particular, it is shown that eukaryotic nucleo-cytoplasmic large DNA viruses, including poxviruses, asfarviruses, iridoviruses, phycodnaviruses and the mimivirus, encode AEPs of a distinct family, which also includes the herpesvirus primases whose relationship to AEPs has not been recognized previously. Many eukaryotic genomes, including chordates and plants, encode previously uncharacterized homologs of these predicted viral primases, which might be involved in novel DNA repair pathways. At a deeper level of evolutionary connections, structural comparisons indicate that AEPs, the nucleases involved in the initiation of rolling circle replication in plasmids and viruses, and origin-binding domains of papilloma and polyoma viruses evolved from a common ancestral protein that might have been involved in a protein-priming mechanism of initiation of DNA replication. Contextual analysis of multidomain protein architectures and gene neighborhoods in prokaryotes and viruses reveals remarkable parallels between AEPs and the unrelated DnaG-type primases, in particular, tight associations with the same repertoire of helicases. These observations point to a functional equivalence of the two classes of primases, which seem to have repeatedly displaced each other in various extrachromosomal replicons.
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影响因子：
6.8
作者：
Aravind, L;Mazumder, R;Koonin, EV
通讯作者：
Koonin, EV
影响因子：
13.8
作者：
Dandekar, T;Snel, B;Bork, P
通讯作者：
Bork, P
影响因子：
56.9
作者：
Della, M;Palmbos, PL;Doherty, AJ
通讯作者：
Doherty, AJ
影响因子：
14.9
作者：
BRAITHWAITE, DK;ITO, J
通讯作者：
ITO, J
影响因子：
5.8
作者：
Cuff, JA;Clamp, ME;Barton, GJ
通讯作者：
Barton, GJ