Transcription factor and bone marrow stromal cells in osseointegration of dental implants.
Transcription factor and bone marrow stromal cells in osseointegration of dental implants.
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DOI：
10.22203/ecm.v026a19
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发表时间：
2013-12-19
影响因子：
3.1
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Yan SG;Zhang J;Tu Q;Ye JH;Luo E;Schuler M;Dard MM;Yu Y;Murray D;Cochran DL;Kim SH;Yang P;Chen J
钛种植体广泛应用于牙科诊所和骨科手术。然而，在插入植入物之后，植入物周围的骨形成相对缓慢。本研究评价了骨髓基质细胞（BMSCs）强制表达特异性富含AT序列结合蛋白2（SATB 2）对钛种植体骨整合的影响。为了确定BMSC中SATB 2过表达是否可以增强植入物的骨整合，BMSC被编码Satb 2的逆转录病毒（pBABE-Satb 2）感染，并在将钛植入物植入小鼠股骨之前局部应用于骨缺损。植入后7天和21天，分离股骨进行免疫组织化学（IHC）染色、苏木精伊红（H&E）染色、实时定量逆转录聚合酶链反应（qRT-PCR）和显微计算机断层扫描（μCT）分析。免疫组化染色显示SATB 2高表达的BMSCs在种植体周围的骨组织中呈密集分布。组织学分析显示，SATB 2过表达的BMSCs在植入后3周显著增强了新骨形成和骨与植入物的接触。实时qRT-PCR结果显示，局部递送SATB 2过表达的BMSC增强了植入部位中有效成骨转录因子和骨基质蛋白的表达水平。μCT分析表明，术后3周，过表达SATB 2的BMSC显着增加了植入物周围新形成的骨密度。这些结果得出结论，局部递送SATB 2过表达的BMSC显著加速钛植入物的骨整合。这些结果为SATB 2的未来药理学和临床应用提供了支持，它可以加速钛种植体周围的骨再生。
Titanium implants are widely used in dental clinics and orthopaedic surgery. However, bone formation surrounding the implant is relatively slow after inserting the implant. The current study assessed the effects of bone marrow stromal cells (BMSCs) with forced expression of special AT-rich sequence-binding protein 2 (SATB2) on the osseointegration of titanium implants. To determine whether SATB2 overexpression in BMSCs can enhance the osseointegration of implants, BMSCs were infected with the retrovirus encoding Satb2 (pBABE-Satb2) and were locally applied to bone defects before implanting the titanium implants in the mouse femur. Seven and twenty-one days after implantation, the femora were isolated for immunohistochemical (IHC) staining, haematoxylin eosin (H&E) staining, real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), and micro-computed tomography (μCT) analysis. IHC staining analysis revealed that SATB2-overexpressing BMSCs were intensely distributed in the bone tissue surrounding the implant. Histological analysis showed that SATB2-overexpressing BMSCs significantly enhanced new bone formation and bone-to-implant contact 3 weeks after implantation. Real-time qRT-PCR results showed that the local delivery of SATB2-overexpressing BMSCs enhanced expression levels of potent osteogenic transcription factors and bone matrix proteins in the implantation sites. μCT analysis demonstrated that SATB2-overexpressing BMSCs significantly increased the density of the newly formed bone surrounding the implant 3 weeks postoperatively. These results conclude that local delivery of SATB2-overexpressing BMSCs significantly accelerates osseointegration of titanium implants. These results provide support for future pharmacological and clinical applications of SATB2, which accelerates bone regeneration around titanium implants.
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影响因子：
14
作者：
Yan, S. G.;Zhang, J.;Tu, Q. S.;Ye, J. H.;Luo, E.;Schuler, M.;Kim, M. S.;Griffin, T.;Zhao, J.;Duan, X. J.;Cochran, D. J.;Murray, D.;Yang, P. S.;Chen, J.
通讯作者：
Chen, J.
影响因子：
8
作者：
Mushahary D;Sravanthi R;Li Y;Kumar MJ;Harishankar N;Hodgson PD;Wen C;Pande G
通讯作者：
Pande G
影响因子：
5
作者：
Traini, T.;Mangano, C.;Piattelli, A.
通讯作者：
Piattelli, A.
影响因子：
4.9
作者：
Schneider, GB;Zaharias, R;Stanford, C
通讯作者：
Stanford, C
影响因子：
4.1
作者：
Zhang, Jin;Tu, Qisheng;Chen, Jake
通讯作者：
Chen, Jake