Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma-enteric isoform in differentiating striated myocytes.
Cellular distribution of smooth muscle actins during mammalian embryogenesis: expression of the alpha-vascular but not the gamma-enteric isoform in differentiating striated myocytes.
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DOI：
10.1083/jcb.109.6.2929
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发表时间：
1989-12
期刊：
影响因子：
--
通讯作者：
Lessard JL
中科院分区：
文献类型：
--
作者：
Sawtell NM;Lessard JL
通过使用肌动蛋白同工酶特异性单克隆抗体原位抗原定位，分析了从妊娠第8天到新生第1周大鼠胚胎中α-血管和γ-肠平滑肌肌动蛋白同工酶的细胞分布。α-血管肌动蛋白同工型在gd 10时首次在胚胎血管的离散细胞中被检测到。到gd 14，这种亚型也出现在发育中的气道和肠道周围的间充质细胞内层。然而，直到gd 15，当用γ-肠特异性探针标记发育中的主动脉、气道和肠道周围的细胞时，才检测到γ-肠肌动蛋白。这两种肌动蛋白亚型在发育中的平滑肌区域持续表达，通过妊娠剩余时间和新生儿第一周，此时它们的分布与成人中发现的一致。除了发育中的平滑肌，α-血管肌动蛋白亚型在分化中的横纹肌细胞中表达。在妊娠第10天，在发育中的心肌细胞中，α-血管特异性探针有强烈的标记，在24小时内，在体节肌节细胞中。尽管在出生后第17天，横纹肌细胞中存在显著水平的平滑肌肌动蛋白，但在出生后第一周结束时，在心肌或骨骼肌中不再检测到α-血管肌动蛋白。因此，横纹肌细胞的正常发育顺序包括α-血管平滑肌肌动蛋白亚型的瞬时表达。与此相反，γ-肠平滑肌肌动蛋白在胚胎横纹肌中没有检测到在任何时间。这两种平滑肌亚型的出现和分布的不同时间表明它们的表达在发育过程中受到独立调节。
The cellular distribution of the alpha-vascular and gamma-enteric smooth muscle actin isoforms was analyzed in rat embryos from gestational day (gd) 8 through the first neonatal week by in situ antigen localization using isoactin specific monoclonal antibodies. The alpha-vascular actin isoform was first detected on gd 10 in discrete cells lining the embryonic vasculature. By gd 14, this isoform was also present in the inner layers of mesenchymal cells condensing around the developing airways and gut. The gamma-enteric actin, however, was not detected until gd 15 when cells surrounding the developing aorta, airways, and gut labeled with the gamma-enteric-specific probe. There was continued expression of these two actin isoforms in regions of developing smooth muscle through the remainder of gestation and first neonatal week at which time their distribution coincided with that found in the adult. In addition to developing smooth muscle, the alpha- vascular actin isoform was expressed in differentiating striated muscle cells. On gd 10, there was intense labeling with the alpha-vascular specific probe in developing myocardiocytes and, within 24 h, in somitic myotomal cells. Although significant levels of this smooth muscle actin were present in striated myocytes through gd 17, by the end of the first postnatal week, alpha-vascular actin was no longer detectable in either cardiac or skeletal muscle. Thus, the normal developmental sequence of striated muscle cells includes the transient expression of the alpha-vascular smooth muscle actin isoform. In contrast, the gamma-enteric smooth muscle actin was not detected at any time in embryonic striated muscle. The differential timing of appearance and distribution of these two smooth muscle isoforms indicates that their expression is independently regulated during development.
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影响因子：
5.3
作者：
MCHUGH, KM;LESSARD, JL
通讯作者：
LESSARD, JL
DOI：
10.1083/jcb.106.6.2077
发表时间：
1988-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hayward LJ;Zhu YY;Schwartz RJ
通讯作者：
Schwartz RJ
影响因子：
2.7
作者：
ORDAHL, CP
通讯作者：
ORDAHL, CP
影响因子：
2.7
作者：
TRAEGER, L;MACKENZIE, JM;GOLDSTEIN, MA
通讯作者：
GOLDSTEIN, MA
影响因子：
2.9
作者：
VANDEKERCKHOVE, J;WEBER, K
通讯作者：
WEBER, K