Inhalation of ultrafine particles alters blood leukocyte expression of adhesion molecules in humans.
Inhalation of ultrafine particles alters blood leukocyte expression of adhesion molecules in humans.
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DOI：
10.1289/ehp.7962
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发表时间：
2006-01
影响因子：
10.4
通讯作者：
Utell MJ
中科院分区：
文献类型：
--
作者：
Frampton MW;Stewart JC;Oberdörster G;Morrow PE;Chalupa D;Pietropaoli AP;Frasier LM;Speers DM;Cox C;Huang LS;Utell MJ
超细颗粒（UFP；空气动力学直径 < 100 nm）可能会导致与颗粒空气污染相关的呼吸系统和心血管疾病的发病率和死亡率。我们测试了这样的假设：吸入碳 UFP 对健康和哮喘受试者具有血管效应，可以通过血液白细胞粘附分子表达的变化来检测。健康受试者以三种不同的方案吸入过滤空气和新鲜生成的元素碳颗粒（计数中值直径约 25 nm，几何标准差约 1.6）2 小时：休息时 10 μg/m3、运动时 10 和 25 μg/m3、运动时 50 μg/m3。在第四个方案中，哮喘受试者在运动时吸入空气和 10 μg/m3 UFP。在暴露前和暴露后每隔一段时间采集外周静脉血，并使用多参数流式细胞术定量表面标记物的白细胞表达。在健康受试者中，运动时接触颗粒会降低单核细胞上粘附分子 CD54 和 CD18 以及粒细胞上 CD18 和 CD49d 的表达。血液单核细胞、嗜碱性粒细胞和嗜酸性粒细胞也出现浓度相关的减少，活化标记 CD25 的淋巴细胞表达增加。在哮喘受试者中，运动暴露于 10 μg/m3 UFP 会降低单核细胞和嗜酸性粒细胞上的 CD11b 以及粒细胞上的 CD54 表达。颗粒暴露还降低了 CD4+ T 细胞、嗜碱性粒细胞和嗜酸性粒细胞的百分比。吸入元素碳 UFP 会改变外周血白细胞分布和粘附分子的表达，其模式与肺血管床中白细胞滞留增加一致。
Ultrafine particles (UFPs; aerodynamic diameter < 100 nm) may contribute to the respiratory and cardiovascular morbidity and mortality associated with particulate air pollution. We tested the hypothesis that inhalation of carbon UFPs has vascular effects in healthy and asthmatic subjects, detectable as alterations in blood leukocyte expression of adhesion molecules. Healthy subjects inhaled filtered air and freshly generated elemental carbon particles (count median diameter ~ 25 nm, geometric standard deviation ~ 1.6), for 2 hr, in three separate protocols: 10 μg/m3 at rest, 10 and 25 μg/m3 with exercise, and 50 μg/m3 with exercise. In a fourth protocol, subjects with asthma inhaled air and 10 μg/m3 UFPs with exercise. Peripheral venous blood was obtained before and at intervals after exposure, and leukocyte expression of surface markers was quantitated using multiparameter flow cytometry. In healthy subjects, particle exposure with exercise reduced expression of adhesion molecules CD54 and CD18 on monocytes and CD18 and CD49d on granulocytes. There were also concentration-related reductions in blood monocytes, basophils, and eosinophils and increased lymphocyte expression of the activation marker CD25. In subjects with asthma, exposure with exercise to 10 μg/m3 UFPs reduced expression of CD11b on monocytes and eosinophils and CD54 on granulocytes. Particle exposure also reduced the percentage of CD4+ T cells, basophils, and eosinophils. Inhalation of elemental carbon UFPs alters peripheral blood leukocyte distribution and expression of adhesion molecules, in a pattern consistent with increased retention of leukocytes in the pulmonary vascular bed.
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影响因子：
2.1
作者：
GAVRAS, JB;FRAMPTON, MW;UTELL, MJ
通讯作者：
UTELL, MJ
DOI：
10.1016/0090-6980(94)90073-6
发表时间：
1994-01-01
期刊：
PROSTAGLANDINS
影响因子：
--
作者：
LESLIE, CA;DUBEY, DP
通讯作者：
DUBEY, DP
影响因子：
10.4
作者：
Lipsett M;Hurley S;Ostro B
通讯作者：
Ostro B
影响因子：
2.1
作者：
Daigle, CC;Chalupa, DC;Frampton, MW
通讯作者：
Frampton, MW
影响因子：
2.1
作者：
OBERDORSTER, G;GELEIN, RM;WEISS, B
通讯作者：
WEISS, B