Widespread occurrence of expressed fungal secretory peroxidases in forest soils.
Widespread occurrence of expressed fungal secretory peroxidases in forest soils.
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DOI：
10.1371/journal.pone.0095557
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hofrichter M
中科院分区：
文献类型：
--
作者：
Kellner H;Luis P;Pecyna MJ;Barbi F;Kapturska D;Krüger D;Zak DR;Marmeisse R;Vandenbol M;Hofrichter M
真菌分泌型过氧化物酶在植物生物量的转化和降解中介导基本的生态功能。这些酶中的许多对芳香族化合物具有强氧化活性，并且参与植物细胞壁（木质素）和腐殖质的降解。它们包括三大类：II类过氧化物酶（包括木质素过氧化物酶、锰过氧化物酶、通用过氧化物酶和通用过氧化物酶）、染料脱色过氧化物酶和血红素-硫醇过氧化物酶（例如非特异性/芳香族过氧化物酶、氯过氧化物酶）。在这里，我们反复观察到所有主要过氧化物酶组在一系列森林生态系统（例如水青冈属，云杉属，槭属，栎属和杨属）的叶和针叶凋落物中的广泛表达，广泛分布于欧洲和北美。锰过氧化物酶和非特异性过氧化物酶被发现在所有9个调查的森林网站，染料脱色过氧化物酶中观察到的9个网站，从而表明这些酶的真菌生理和生态系统过程的生物学意义。选择分泌型过氧化物酶基因的转录本进行了分析，在几个凋落物分解物种和其他真菌的纯培养物。利用这些信息，我们能够匹配，在环境凋落物样品中，两个锰过氧化物酶序列的小菇galopus和小菇epipterygia和一个非特异性的过氧化物酶转录小菇galopus，这表明了一个重要的作用，这种垃圾和粗木质碎片居住属凋落物芳香和有机物形成的解体和转化。
Fungal secretory peroxidases mediate fundamental ecological functions in the conversion and degradation of plant biomass. Many of these enzymes have strong oxidizing activities towards aromatic compounds and are involved in the degradation of plant cell wall (lignin) and humus. They comprise three major groups: class II peroxidases (including lignin peroxidase, manganese peroxidase, versatile peroxidase and generic peroxidase), dye-decolorizing peroxidases, and heme-thiolate peroxidases (e.g. unspecific/aromatic peroxygenase, chloroperoxidase). Here, we have repeatedly observed a widespread expression of all major peroxidase groups in leaf and needle litter across a range of forest ecosystems (e.g. Fagus, Picea, Acer, Quercus, and Populus spp.), which are widespread in Europe and North America. Manganese peroxidases and unspecific peroxygenases were found expressed in all nine investigated forest sites, and dye-decolorizing peroxidases were observed in five of the nine sites, thereby indicating biological significance of these enzymes for fungal physiology and ecosystem processes. Transcripts of selected secretory peroxidase genes were also analyzed in pure cultures of several litter-decomposing species and other fungi. Using this information, we were able to match, in environmental litter samples, two manganese peroxidase sequences to Mycena galopus and Mycena epipterygia and one unspecific peroxygenase transcript to Mycena galopus, suggesting an important role of this litter- and coarse woody debris-dwelling genus in the disintegration and transformation of litter aromatics and organic matter formation.
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影响因子：
3.8
作者：
Fischer, Markus;Bossdorf, Oliver;Weisser, Wolfgang W.
通讯作者：
Weisser, Wolfgang W.
影响因子：
3.4
作者：
Hofrichter, M
通讯作者：
Hofrichter, M
影响因子：
3.7
作者：
Gröbe G;Ullrich R;Pecyna MJ;Kapturska D;Friedrich S;Hofrichter M;Scheibner K
通讯作者：
Scheibner K
影响因子：
11
作者：
Bodeker, Inga T. M.;Nygren, Cajsa M. R.;Lindahl, Bjorn D.
通讯作者：
Lindahl, Bjorn D.
影响因子：
2.7
作者：
Jee, S. H.;Kim, Y. J.;Ko, S. O.
通讯作者：
Ko, S. O.