Bacteria localization and chorion thinning among preterm premature rupture of membranes.
Bacteria localization and chorion thinning among preterm premature rupture of membranes.
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DOI：
10.1371/journal.pone.0083338
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Murtha AP
中科院分区：
文献类型：
--
作者：
Fortner KB;Grotegut CA;Ransom CE;Bentley RC;Feng L;Lan L;Heine RP;Seed PC;Murtha AP
细菌在胎膜中的定植及其在胎膜破裂发病机制中的作用尚不清楚。先前的回顾性研究显示，未足月胎膜早破（PPROM）受试者的绒毛膜层变薄。我们的目的是前瞻性地检查胎膜早破、早产和足月受试者中的胎膜绒毛膜变薄，并与细菌存在相关。前瞻性地从PPROM = 14、早产（PTL = 8）、早产未分娩（PTNL= 8）、足月分娩（TL = 10）和足月未分娩（TNL = 8）受试者中收集配对的胎膜样本（胎膜破裂和胎膜远端）。 切片用细胞角蛋白进行探测，以使用免疫组织化学鉴定绒毛膜的胎儿滋养层。荧光原位杂交采用宽范围的16s核糖体RNA探针。评估图像，测量绒毛膜和绒毛膜蜕膜，并对细菌荧光进行评分。使用Student t检验、线性混合效应模型和Poisson回归模型（SAS卡里，NC）在组间和组间比较绒毛膜变薄和细菌存在。在所有组中，与远端部位相比，破裂处的胎儿绒毛膜细胞层更薄（147.2 vs. 253.7 µm，p<0.0001）。此外，与所有其他组合并相比，PPROM受试者的绒毛膜变薄程度最大，与采样中心无关[PPROM（114.9）vs. PTL（246.0）vs. PTNL（200.8）vs. TL（217.9）vs. TNL（246.5）]。与所有其他组相比，PPROM受试者的细菌计数最高，无论采样部位或组织学感染如何[PPROM（31）vs. PTL（9）vs. PTNL（7）vs. TL（7）vs. TNL（6）]。在两个研究中心的所有受试者中，细菌计数与绒毛膜变薄呈负相关，甚至排除组织学绒毛膜炎（p<0.0001和p = 0.05）。 与远处相比，破裂部位的胎儿绒毛膜均匀较薄。在PPROM胎儿绒毛膜，我们表现出明显的全球变薄。虽然原因或后果是不确定的，细菌的存在是最大的，并与绒毛膜变薄PPROM受试者呈负相关。
Bacterial colonization of the fetal membranes and its role in pathogenesis of membrane rupture is poorly understood. Prior retrospective work revealed chorion layer thinning in preterm premature rupture of membranes (PPROM) subjects. Our objective was to prospectively examine fetal membrane chorion thinning and to correlate to bacterial presence in PPROM, preterm, and term subjects. Paired membrane samples (membrane rupture and membrane distant) were prospectively collected from: PPROM = 14, preterm labor (PTL = 8), preterm no labor (PTNL = 8), term labor (TL = 10), and term no labor (TNL = 8), subjects. Sections were probed with cytokeratin to identify fetal trophoblast layer of the chorion using immunohistochemistry. Fluorescence in situ hybridization was performed using broad range 16 s ribosomal RNA probe. Images were evaluated, chorion and choriodecidua were measured, and bacterial fluorescence scored. Chorion thinning and bacterial presence were compared among and between groups using Student's t-test, linear mixed effect model, and Poisson regression model (SAS Cary, NC). In all groups, the fetal chorion cellular layer was thinner at rupture compared to distant site (147.2 vs. 253.7 µm, p<0.0001). Further, chorion thinning was greatest among PPROM subjects compared to all other groups combined, regardless of site sampled [PPROM(114.9) vs. PTL(246.0) vs. PTNL(200.8) vs. TL(217.9) vs. TNL(246.5)]. Bacteria counts were highest among PPROM subjects compared to all other groups regardless of site sampled or histologic infection [PPROM(31) vs. PTL(9) vs. PTNL(7) vs. TL(7) vs. TNL(6)]. Among all subjects at both sites, bacterial counts were inversely correlated with chorion thinning, even excluding histologic chorioamnionitis (p<0.0001 and p = 0.05). Fetal chorion was uniformly thinner at rupture site compared to distant sites. In PPROM fetal chorion, we demonstrated pronounced global thinning. Although cause or consequence is uncertain, bacterial presence is greatest and inversely correlated with chorion thinning among PPROM subjects.
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DOI：
10.1111/j.1471-0528.1994.tb11908.x
发表时间：
1994-05-01
期刊：
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY
影响因子：
--
作者：
MALAK, TM;BELL, SC
通讯作者：
BELL, SC
影响因子：
9.8
作者：
Murtha, Amy P.;Nieves, Angel;Heine, R. Phillips
通讯作者：
Heine, R. Phillips
影响因子：
9.8
作者：
Arikat, S;Novince, RW;Moore, JJ
通讯作者：
Moore, JJ
影响因子：
3.6
作者：
El Khwad, M;Stetzer, B;Moore, JJ
通讯作者：
Moore, JJ
影响因子：
3.7
作者：
Jones HE;Harris KA;Azizia M;Bank L;Carpenter B;Hartley JC;Klein N;Peebles D
通讯作者：
Peebles D