Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy.
Treatment with Tang-luo-ning altered the microRNA expression profile in rats with diabetic peripheral neuropathy.
复制标题
糖络宁治疗改变糖尿病周围神经病变大鼠的 microRNA 表达谱
DOI：
10.1080/21655979.2020.1797282
复制
发表时间：
2020-12
期刊：
影响因子：
4.9
通讯作者：
Zhang T
中科院分区：
文献类型：
--
作者：
Li Y;Gao Y;Gong Y;Guo Y;Wang L;Liu Q;Chen F;Zhang T
汤络宁（TLN）是一种治疗糖尿病周围神经病变（DPN）的传统中药；然而，潜在的机制仍不清楚。本研究旨在探讨TLN治疗糖尿病大鼠的microRNA （miRNA）表达谱，并对靶基因进行预测。雄性Sprague-Dawley大鼠随机分为对照组、糖尿病组和tln治疗糖尿病组。用链脲佐菌素诱导糖尿病，给予TLN （5 g/kg/天）口服，连续8周。然后，采集坐骨神经进行miRNA芯片分析。分析差异表达的mirna及其靶基因。与对照组相比，糖尿病大鼠坐骨神经中有24个mirna显著上调，59个mirna下调2倍以上（均P < 0.05）。与未TLN处理的糖尿病大鼠相比，TLN处理的糖尿病大鼠mirna上调26个，下调14个（均P < 0.05）。dpn诱导的miRNA谱改变被TLN处理逆转。共筛选到1402个靶基因。在氧化石墨烯分析中，定位、细胞质和蛋白结合过程中的基因被富集，富集最显著的途径包括神经营养因子、Fc epsilon RI和Wnt信号通路。进一步分析表明，DVL1和NTF3基因参与了这些途径。我们的研究结果表明，TLN可能通过作用于DVL1和NTF3基因来影响Wnt和神经营养因子通路。图形抽象
ABSTRACT Tang-luo-ning (TLN) is a traditional Chinese herbal recipe that has been used to treat diabetic peripheral neuropathy (DPN); nevertheless, the underlying mechanism remains unclear. This study was aimed to investigate the microRNA (miRNA) expression profile in diabetic rats treated with TLN, and the target genes were predicted. Male Sprague-Dawley rats were randomly divided into control, diabetes, and TLN-treated diabetes groups. Diabetes was induced with streptozotocin, and TLN (5 g/kg/day) was orally given for eight weeks. Then, the sciatic nerves were harvested for miRNA microarray analyses. The differentially expressed miRNAs and their target genes were analyzed. Compared with the control rats, 24 miRNAs were significantly upregulated, and 59 were downregulated in the sciatic nerves of the diabetic rats by more than two folds (all P < 0.05). In TLN-treated diabetes rats, 26 miRNAs were upregulated, and 14 were downregulated compared with diabetic rats without TLN treatment (all P < 0.05). DPN-induced alterations of the miRNA profile were reversed by the TLN treatment. A total of 1402 target genes were screened. In GO analysis, genes in localization, cytoplasm, and protein binding processes were enriched, and the most significantly enriched pathways included the neurotrophin, Fc epsilon RI, and Wnt signaling pathways. Further analyses revealed that DVL1 and NTF3 genes were involved in these pathways. Our findings indicate that TLN may affect the Wnt and neurotrophin pathways by acting on DVL1 and NTF3 genes. GRAPHICAL ABSTRACT
登录
查看更多内容
糖络宁是一种中药，通过调节 PERK/Nrf2 通路减轻体内和体外糖尿病周围神经病变，
影响因子：
4.6
作者：
Yang, Xinwei;Yao, Weijie;Xu, Liping
通讯作者：
Xu, Liping
DOI：
10.1016/j.jtcvs.2017.07.073
发表时间：
2018-03-01
影响因子：
6
作者：
Duan, Xiaoxia;Zhu, Tao;Wang, Xiaobin
通讯作者：
Wang, Xiaobin
影响因子：
4.4
作者：
Li, Liqi;Gong, Zhaoyong;Li, Liya
通讯作者：
Li, Liya
影响因子：
2.5
作者：
Song, Jihwan;Lee, Soon-Tae;Kim, Manho
通讯作者：
Kim, Manho
DOI：
10.1016/s1006-706x(09)60036-4
发表时间：
2009-05-01
影响因子：
2.5
作者：
Tian Zhi-hong;Li Ben-hai;Jiang Zhong-hang
通讯作者：
Jiang Zhong-hang