Lipidomics analysis reveals efficient storage of hepatic triacylglycerides enriched in unsaturated fatty acids after one bout of exercise in mice.
Lipidomics analysis reveals efficient storage of hepatic triacylglycerides enriched in unsaturated fatty acids after one bout of exercise in mice.
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脂质组学分析表明，小鼠进行一轮运动后，富含不饱和脂肪酸的肝脏三酰甘油酯可以有效储存
DOI：
10.1371/journal.pone.0013318
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发表时间：
2010-10-13
期刊：
影响因子：
3.7
通讯作者：
Weigert C
中科院分区：
文献类型：
--
作者：
Hu C;Hoene M;Zhao X;Häring HU;Schleicher E;Lehmann R;Han X;Xu G;Weigert C
背景耐力运动可诱导脂肪分解，增加循环中游离脂肪酸(FFA)的浓度，并促进肌肉对脂肪酸的摄取和氧化。关于运动期间和运动后肝脏中脂代谢的调节，人们知之甚少。方法/主要研究结果C57BL/6J小鼠在中等强度的跑台上跑了60分钟，恢复3小时后，立即进行了基于超快速液相色谱-质谱仪(UFLC-MS)的脂肪组学分析。115种定量脂质分子的偏最小二乘-DA计分图显示，久坐小鼠和恢复小鼠的肝脏脂质谱明显分离，但跑步后小鼠的肝脏脂质谱不是立即分离的。21种脂类被认为是造成肝脏脂质谱差异的最主要原因，包括17种三酰甘油(TG)、1种溶血磷脂酰胆碱(LPC)和3种磷脂酰胆碱(PC)。恢复期TG物种较多，PC物种较少。单个TG物种的积累程度与理论储存的能量量有很好的相关性，而只含有饱和脂肪酸或单一不饱和脂肪酸的TG物种没有发现增加。肝脏总甘油三酯含量在恢复期增加到163%，而骨骼肌中甘油三酯含量在运动后显著下降，在恢复期保持较低水平。禁食和再喂食小鼠的结果表明，禁食诱导的脂肪分解与肝脏TG的显著积聚有关，而再喂食5h则相反。因此，食物本身并不会升高肝脏的TG。结论耐力运动或禁食诱导的高FFA利用率导致肝脏TG短暂蓄积，而运动时肌肉TG含量下降可能是由于肌肉脂肪酸氧化增加所致。
Background Endurance exercise induces lipolysis, increases circulating concentrations of free fatty acids (FFA) and the uptake and oxidation of fatty acids in the working muscle. Less is known about the regulation of lipid metabolism in the liver during and post-exercise. Methodology/Principal Findings We performed an ultra fast liquid chromatography-mass spectrometry (UFLC-MS) based lipidomics analysis of liver tissue samples obtained from C57Bl/6J mice immediately after a 60 min treadmill run of moderate intensity, and after 3 h of recovery. The PLS-DA scores plot for 115 quantified lipid molecular species revealed a clear separation of the hepatic lipid profile of sedentary from recovering mice, but not from mice immediately after running. 21 lipid species were considered to be most responsible for the difference in the hepatic lipid profiles, including 17 triacylglycerides (TG), one lysophosphatidylcholine (LPC) and three phosphatidylcholines (PC). TG species were found to be more abundant in the recovery phase, while PC species were decreased. The degree of accumulation of individual TG species correlated well with the amount of theoretical energy stored whereas no increase was found for TG species containing only saturated or one monounsaturated fatty acid. Total liver TG content as assayed by an enzymatic method was increased to 163% in the recovery phase, while it was significantly decreased in skeletal muscle by the exercise bout and remained less in the recovery phase. Results from fasted and refed mice indicate that fasting-induced lipolysis was associated with a pronounced accumulation of hepatic TG, which is reversed by refeeding for 5 h. Thus food intake per se did not elevate hepatic TG. Conclusion These data indicate that high availability of FFA induced by endurance exercise or fasting resulted in a transient hepatic TG accumulation, while muscle TG content was decreased during exercise presumably due to increased muscle fatty acid oxidation.
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