Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids.
Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids.
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蚜虫气味结合蛋白和化学感应蛋白的综合转录组学和基因组分析
DOI：
10.1111/imb.12513
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发表时间：
2019-03
影响因子：
2.6
通讯作者：
Gu SH
中科院分区：
文献类型：
--
作者：
Wang Q;Zhou JJ;Liu JT;Huang GZ;Xu WY;Zhang Q;Chen JL;Zhang YJ;Li XC;Gu SH
气味结合蛋白（OBP）和化学感受蛋白（CSP）在昆虫化学感受识别中起着重要作用。在这里，我们从桃蚜转录组和基因组中鉴定出9个OBP和9个CSP。基因组结构分析表明，OBP基因的内含子数量和长度都很高，这似乎是蚜虫OBP基因的一个独特特征。桃蚜OBP基因（OBP 3/7/8）与CSP 1/4/6、CSP 2/9和CSP 5/8在基因组中串联排列。五种不同的蚜虫物种的系统发育分析表明，蚜虫OBP和CSP是保守的单拷贝在所有的蚜虫（偶尔的损失），表明每个OBP和CSP类进化从一个单一的基因在蚜虫的共同祖先没有随后的重复。模体模式分析表明，蚜虫OBP和CSP模体高度保守，这可能表明蚜虫OBP和CSP的保守功能。三个OBP（MperOBP 6/7/10）是触角特异性表达的，五个OBP（MperOBP 2/4/5/8/9）是触角富集表达的，与它们假定的嗅觉作用一致。桃蚜CSP在非感觉器官中的表达谱比OBP广泛得多。9个MperCSPs中没有一个被发现是触角特异性的，但其中5个（MperCSP 1/2/4/5/6）在腿中的表达水平高于其他组织。MperCSP 10主要在触角和腿中表达。桃蚜CSPs广泛而多样的表达模式表明其在嗅觉感知、发育等过程中具有多种功能。
Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play essential roles in insect chemosensory recognition. Here, we identified nine OBPs and nine CSPs from the Myzus persicae transcriptome and genome. Genomic structure analysis showed that the number and length of the introns are much higher, and this appears to be a unique feature of aphid OBP genes. Three M. persicae OBP genes (OBP3/7/8) as well as CSP1/4/6, CSP2/9 and CSP5/8 are tandem arrayed in the genome. Phylogenetic analyses of five different aphid species suggest that aphid OBPs and CSPs are conserved in single copy across all aphids (with occasional losses), indicating that each OBP and CSP class evolved from a single gene in the common ancestor of aphids without subsequent duplication. Motif pattern analysis revealed that aphid OBP and CSP motifs are highly conserved, and this could suggest the conserved functions of aphid OBPs and CSPs. Three OBPs (MperOBP6/7/10) are expressed antennae specifically, and five OBPs (MperOBP2/4/5/8/9) are expressed antennae enriched, consistent with their putative olfactory roles. M. persicae CSPs showed much broader expression profiles in nonsensory organs than OBPs. None of the nine MperCSPs were found to be antennae specific, but five of them (MperCSP1/2/4/5/6) showed higher expression levels in the legs than in other tissues. MperCSP10 mainly expressed in the antennae and legs. The broad and diverse expression patterns of M. persicae CSPs suggest their multifunctions in olfactory perception, development and other processes.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3
作者：
Löytynoja A;Goldman N
通讯作者：
Goldman N
DOI：
10.1046/j.1432-1327.1999.00438.x
发表时间：
1999-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Angeli, S;Ceron, F;Pelosi, P
通讯作者：
Pelosi, P
识别与蚜虫 Rhopalosiphum padi 中 (E)-β-法尼烯感知相关的种内警报信息素和两种保守的气味结合蛋白
影响因子：
2.2
作者：
Fan, Jia;Xue, Wenxin;Chen, Julian
通讯作者：
Chen, Julian
在 Adelphocoris suturalis Jakovlev 的触角转录组中鉴定出气味结合和化学感应蛋白
DOI：
10.1016/j.cbd.2016.03.001
发表时间：
2017-12-01
影响因子：
3
作者：
Cui, Huan-Huan;Gu, Shao-Hua;Zhang, Yong-Jun
通讯作者：
Zhang, Yong-Jun