Effects of age, sex, and persistent organic pollutants on DNA methylation in children.
Effects of age, sex, and persistent organic pollutants on DNA methylation in children.
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DOI：
10.1002/em.21845
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发表时间：
2014-04
影响因子：
2.8
通讯作者：
Holland, Nina
中科院分区：
文献类型：
--
作者：
Huen, Karen;Yousefi, Paul;Bradman, Asa;Yan, Liying;Harley, Kim G.;Kogut, Katherine;Eskenazi, Brenda;Holland, Nina
DNA甲基化等表观遗传变化可能是环境暴露影响健康的分子机制。Alu和长散布核苷酸元件（LINE-1）的甲基化是流行病学研究中经常使用的DNA甲基化的公认测量方法。然而，很少有研究探讨了宿主因素对儿童LINE-1和Alu甲基化的影响。我们的特点的关系，年龄，性别和产前接触持久性有机污染物（POP），二氯二苯三氯乙烷（DDT），二氯二苯二氯乙烯（DDE），和多溴联苯醚（PBDEs），与DNA甲基化在出生队列的墨西哥裔美国儿童参加CHAMACOS研究。我们通过焦磷酸测序从新生儿和9岁儿童（n=358）收集的全血样品中分离的亚硫酸氢盐处理的DNA来测量Alu和LINE-1甲基化。持久性有机污染物测量在怀孕后期的母亲血清。9岁奥尔兹的DNA甲基化水平低于新生儿，男孩高于女孩。较高的产前DDT/E暴露与出生时较低的Alu甲基化相关，特别是在调整细胞类型组成后（p=0.02，o，p′ -DDT）。持久性有机污染物与LINE-1甲基化之间的关系只有在同时研究了滴滴涕/E与多溴二苯醚的共同接触后才能确定。我们的数据表明，重复元件甲基化可以是年龄和性别表观遗传差异的信息标记，并且产前接触POP可能与胎儿血液中的低甲基化有关。考虑到共同接触不同类型的化学品和调整血细胞类型可能会增加流行病学研究的表观遗传分析的灵敏度。
Epigenetic changes such as DNA methylation may be a molecular mechanism through which environmental exposures affect health. Methylation of Alu and long interspersed nucleotide elements (LINE-1) is a well-established measure of DNA methylation often used in epidemiologic studies. Yet, few studies have examined the effects of host factors on LINE-1 and Alu methylation in children. We characterized the relationship of age, sex, and prenatal exposure to persistent organic pollutants (POPs), dichlorodiphenyl trichloroethane (DDT), dichlorodiphenyldichloroethylene (DDE), and polybrominated diphenyl ethers (PBDEs), with DNA methylation in a birth cohort of Mexican-American children participating in the CHAMACOS study. We measured Alu and LINE-1 methylation by pyrosequencing bisulfite-treated DNA isolated from whole blood samples collected from newborns and 9-year old children (n=358). POPs were measured in maternal serum during late pregnancy. Levels of DNA methylation were lower in 9-year olds compared to newborns and were higher in boys compared to girls. Higher prenatal DDT/E exposure was associated with lower Alu methylation at birth, particularly after adjusting for cell type composition (p=0.02 for o,p′ -DDT). Associations of POPs with LINE-1 methylation were only identified after examining the co-exposure of DDT/E with PBDEs simultaneously. Our data suggest that repeat element methylation can be an informative marker of epigenetic differences by age and sex and that prenatal exposure to POPs may be linked to hypomethylation in fetal blood. Accounting for co-exposure to different types of chemicals and adjusting for blood cell types may increase sensitivity of epigenetic analyses for epidemiological studies.
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影响因子：
3.6
作者：
Baccarelli A;Bollati V
通讯作者：
Bollati V
DOI：
10.1017/s2040174412000104
发表时间：
2012-06
影响因子：
1.7
作者：
Burris, H. H.;Rifas-Shiman, S. L.;Baccarelli, A.;Tarantini, L.;Boeke, C. E.;Kleinman, K.;Litonjua, A. A.;Rich-Edwards, J. W.;Gillman, M. W.
通讯作者：
Gillman, M. W.
影响因子：
10.4
作者：
Gascon, Mireia;Fort, Marta;Vrijheid, Martine
通讯作者：
Vrijheid, Martine
影响因子：
11.2
作者：
Bollati, Valentina;Baccarelli, Andrea;Yang, Allen S.
通讯作者：
Yang, Allen S.
年轻女性的DDT和乳腺癌：有关暴露年龄的重要性的新数据。
影响因子：
10.4
作者：
Cohn BA;Wolff MS;Cirillo PM;Sholtz RI
通讯作者：
Sholtz RI