An experimental model for studying the biomechanics of embryonic tendon: Evidence that the development of mechanical properties depends on the actinomyosin machinery.
An experimental model for studying the biomechanics of embryonic tendon: Evidence that the development of mechanical properties depends on the actinomyosin machinery.
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DOI：
10.1016/j.matbio.2010.08.009
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发表时间：
2010-10
期刊：
影响因子：
6.9
通讯作者：
Kadler, Karl E.
中科院分区：
文献类型：
--
作者：
Kalson, Nicholas S.;Holmes, David F.;Kapacee, Zoher;Otermin, Iker;Lu, Yinhui;Ennos, Roland A.;Canty-Laird, Elizabeth G.;Kadler, Karl E.
关键词：
肌腱将肌肉附着在骨骼上，从而在关节运动过程中传递拉力。然而，由于研究体内组织力学的实际困难，对建立肌腱机械性能的机制的详细了解仍然难以捉摸。在这里，我们对通过在固定长度纤维蛋白凝胶中培养胚胎肌腱细胞而制成的肌腱样结构进行了研究。该结构显示出机械性能（脚趾-线性-失效应力-应变曲线、刚度、极限拉伸强度和失效应变）以及胶原纤维体积分数和细胞外基质（ECM）/细胞比率，这些特性在统计上与胚胎鸡跖腱的相似。当用 Triton X-100（溶解膜）、细胞松弛素（分解肌动蛋白细胞骨架）和肌球蛋白 II（非肌肉肌球蛋白 II 的小分子抑制剂）分别处理构建体时，培养过程中机械特性的发展被消除。重要的是，这些处理对处理前存在的结构的机械性能没有影响。活细胞成像和 14C-脯氨酸代谢标记表明，blebbistatin 抑制构建体的收缩，而不影响细胞活力、前胶原合成或前胶原向胶原的转化。总之，肌腱结构本身的机械性能可归因于细胞产生的 ECM，但培养期间机械性能的改善取决于非肌肉肌球蛋白 II 衍生的力。
Tendons attach muscles to bone and thereby transmit tensile forces during joint movement. However, a detailed understanding of the mechanisms that establish the mechanical properties of tendon has remained elusive because of the practical difficulties of studying tissue mechanics in vivo. Here we have performed a study of tendon-like constructs made by culturing embryonic tendon cells in fixed-length fibrin gels. The constructs display mechanical properties (toe–linear–fail stress–strain curve, stiffness, ultimate tensile strength, and failure strain) as well as collagen fibril volume fraction and extracellular matrix (ECM)/cell ratio that are statistically similar to those of embryonic chick metatarsal tendons. The development of mechanical properties during time in culture was abolished when the constructs were treated separately with Triton X-100 (to solubilise membranes), cytochalasin (to disassemble the actin cytoskeleton) and blebbistatin (a small molecule inhibitor of non-muscle myosin II). Importantly, these treatments had no effect on the mechanical properties of the constructs that existed prior to treatment. Live-cell imaging and 14C-proline metabolic labeling showed that blebbistatin inhibited the contraction of the constructs without affecting cell viability, procollagen synthesis, or conversion of procollagen to collagen. In conclusion, the mechanical properties per se of the tendon constructs are attributable to the ECM generated by the cells but the improvement of mechanical properties during time in culture was dependent on non-muscle myosin II-derived forces.
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影响因子：
4
作者：
Humphries JD;Byron A;Humphries MJ
通讯作者：
Humphries MJ
DOI：
10.1111/j.1749-6632.1985.tb51173.x
发表时间：
1985-12-30
影响因子：
5.2
作者：
BIRK, DE;TRELSTAD, RL
通讯作者：
TRELSTAD, RL
DOI：
10.1083/jcb.103.1.231
发表时间：
1986-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Birk DE;Trelstad RL
通讯作者：
Trelstad RL
DOI：
10.1016/0141-8130(88)90048-7
发表时间：
1988-08-01
影响因子：
8.2
作者：
MCBRIDE, DJ;TRELSTAD, RL;SILVER, FH
通讯作者：
SILVER, FH
影响因子：
16.8
作者：
Allingham, JS;Smith, R;Rayment, I
通讯作者：
Rayment, I