Vision-dependent specification of cell types and function in the developing cortex.
Vision-dependent specification of cell types and function in the developing cortex.
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发育中的皮层细胞类型和功能的视觉依赖性特化。
DOI：
10.1016/j.cell.2021.12.022
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发表时间：
2022-01-20
期刊：
影响因子：
64.5
通讯作者：
Zipursky SL
中科院分区：
文献类型：
--
作者：
Cheng S;Butrus S;Tan L;Xu R;Sagireddy S;Trachtenberg JT;Shekhar K;Zipursky SL
出生后的经验在塑造大脑皮层回路中的作用，虽然长期以来一直受到重视，但在细胞类型的水平上却知之甚少。我们探索这在小鼠初级视觉皮层（V1）使用单核RNA测序，视觉剥夺，遗传学和功能成像。我们发现，视觉选择性地驱动上层（L）（L2/3/4）中神经元能细胞类型的特化，而深层神经元能、GABA能和非神经元细胞类型在睁眼之前建立。L2/3细胞类型形成由约200个基因的分级表达定义的经验依赖性空间连续体，包括细胞粘附和突触形成的调节因子。其中之一，Igsf 9 b，一个视觉依赖性基因编码抑制性突触细胞粘附分子，是所需的正常发展的双眼反应在L2/3。总之，视觉优先通过调节细胞类型特异性基因表达程序来调节上层神经元细胞类型的发育。对发育中的小鼠初级视皮层（V1）进行转录组学、遗传学和功能分析，揭示早期感觉经验通过调节空间和时间基因表达程序选择性地影响第2/3层视皮层能神经元的发育。
The role of postnatal experience in sculpting cortical circuitry, while long appreciated, is poorly understood at the level of cell types. We explore this in the mouse primary visual cortex (V1) using single-nucleus RNA-sequencing, visual deprivation, genetics, and functional imaging. We find that vision selectively drives the specification of glutamatergic cell types in upper layers (L) (L2/3/4), while deeper-layer glutamatergic, GABAergic, and non-neuronal cell types are established prior to eye opening. L2/3 cell types form an experience-dependent spatial continuum defined by the graded expression of ~200 genes, including regulators of cell adhesion and synapse formation. One of these, Igsf9b, a vision-dependent gene encoding an inhibitory synaptic cell adhesion molecule, is required for the normal development of binocular responses in L2/3. In summary, vision preferentially regulates the development of upper-layer glutamatergic cell types through the regulation of cell type-specific gene expression programs. Transcriptomic, genetic, and functional analysis of the developing mouse primary visual cortex (V1) reveals that early sensory experience selectively influences the development of layer 2/3 glutamatergic neurons by regulating spatial and temporal gene expression programs.
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影响因子：
46.9
作者：
Becht, Etienne;McInnes, Leland;Newell, Evan W.
通讯作者：
Newell, Evan W.
影响因子：
25
作者：
Gouwens NW;Sorensen SA;Berg J;Lee C;Jarsky T;Ting J;Sunkin SM;Feng D;Anastassiou CA;Barkan E;Bickley K;Blesie N;Braun T;Brouner K;Budzillo A;Caldejon S;Casper T;Castelli D;Chong P;Crichton K;Cuhaciyan C;Daigle TL;Dalley R;Dee N;Desta T;Ding SL;Dingman S;Doperalski A;Dotson N;Egdorf T;Fisher M;de Frates RA;Garren E;Garwood M;Gary A;Gaudreault N;Godfrey K;Gorham M;Gu H;Habel C;Hadley K;Harrington J;Harris JA;Henry A;Hill D;Josephsen S;Kebede S;Kim L;Kroll M;Lee B;Lemon T;Link KE;Liu X;Long B;Mann R;McGraw M;Mihalas S;Mukora A;Murphy GJ;Ng L;Ngo K;Nguyen TN;Nicovich PR;Oldre A;Park D;Parry S;Perkins J;Potekhina L;Reid D;Robertson M;Sandman D;Schroedter M;Slaughterbeck C;Soler-Llavina G;Sulc J;Szafer A;Tasic B;Taskin N;Teeter C;Thatra N;Tung H;Wakeman W;Williams G;Young R;Zhou Z;Farrell C;Peng H;Hawrylycz MJ;Lein E;Ng L;Arkhipov A;Bernard A;Phillips JW;Zeng H;Koch C
通讯作者：
Koch C
影响因子：
16.6
作者：
Arneson D;Zhang G;Ying Z;Zhuang Y;Byun HR;Ahn IS;Gomez-Pinilla F;Yang X
通讯作者：
Yang X
影响因子：
25
作者：
Hrvatin S;Hochbaum DR;Nagy MA;Cicconet M;Robertson K;Cheadle L;Zilionis R;Ratner A;Borges-Monroy R;Klein AM;Sabatini BL;Greenberg ME
通讯作者：
Greenberg ME
影响因子：
16.2
作者：
Espinosa JS;Stryker MP
通讯作者：
Stryker MP