Effect of concentration and adsorption time on the formation of a large-scale origami pattern

Effect of concentration and adsorption time on the formation of a large-scale origami pattern
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浓度和吸附时间对大尺寸折纸图案形成的影响

DOI:
10.1007/s41365-019-0639-6
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发表时间:
2019-06
影响因子:
2.8
通讯作者:
Li Bin
Li Bin
中科院分区:
物理与天体物理2区
文献类型:
--
作者:
Liu Wen-Jing;Zhang Ping;Sun Tong;Li Lin;Wei Yu-Hui;Wang Kai-Zhe;Liu Lin;Li Bin

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将DNA纳米结构以尽可能高的精度和可控性排列成所需的大规模周期性图案对于DNA在功能生物材料中的应用至关重要;然而,在纳米技术领域中,不利用分子相互作用形成DNA纳米结构图案仍然是困难的。在这篇文章中,我们使用折纸的最佳浓度和吸附时间,在不改变折纸本身的情况下,诱导DNA折纸在云母上形成大规模的2D图案。DNA折纸结构可以通过离子之间的对称和静电相互作用的紧密堆积形成图案,这被原子力显微镜图像证实。此外,我们确定了DNA折纸的浓度和最佳吸附时间的有利条件,这可以使DNA折纸形成图案。这项工作为理解DNA在云母上的吸附提供了一种见解,并指导了对规则DNA纳米结构模式的研究,这些模式可以作为蛋白质或其他生物分子模式形成的模板。
The arrangement of DNA-based nanostructures into the desired large-scale periodic pattern with the highest possible accuracy and control is essential for the DNA application in functional biomaterials; however, formation of a DNA nanostructure pattern without utilizing the molecular interactions in nanotechnology field remains difficult. In this article, we use the optimal concentration and adsorption time of origami to induce DNA origami in the form of a large-scale 2D pattern on mica without changing the origami itself. DNA origami structures can form a pattern by close packing of symmetric and electrostatic interactions between ions, which was confirmed by the atomic force microscopy images. Furthermore, we identified favorable conditions for the concentration of DNA origami and optimal adsorption time, which can enable pattern formation with DNA origami. This work provides an insight to understand the adsorption of DNA on mica and guides researches on regular DNA nanostructure pattern, which can serve as templates for pattern formation of proteins or other biomolecules.
DOI: 10.1038/171964b0
发表时间: 1953-01-01
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