AINTEGUMENTA and AINTEGUMENTA-LIKE6 directly regulate floral homeotic, growth, and vascular development genes in young Arabidopsis flowers.
AINTEGUMENTA and AINTEGUMENTA-LIKE6 directly regulate floral homeotic, growth, and vascular development genes in young Arabidopsis flowers.
复制标题
DOI：
10.1093/jxb/erab223
复制
发表时间：
2021-07-28
影响因子：
6.9
通讯作者：
Loraine AE
中科院分区：
文献类型：
--
作者：
Krizek BA;Bantle AT;Heflin JM;Han H;Freese NH;Loraine AE
拟南芥ANT和AIL 6直接调节参与早期花发育不同方面的基因，包括指定花器官身份的基因以及调节生长和维管发育的基因。拟南芥花原基在四个同心轮的固定位置产生器官原基。每轮花器官原基经历不同的发育程序，成为四种器官类型之一（萼片，花瓣，雄蕊和心皮）。拟南芥转录因子AINTEGUMENTA（ANT）和AINTEGUMENTA-LIKE 6（AIL 6）是花器官起始的正确定位所必需的，有助于花器官身份的规范，并调节发育中的花器官的生长和形态建成。为了深入了解ANT和AIL 6促进花器官发生的分子机制，我们确定了ANT和AIL 6在第3阶段花原基的全基因组结合位点，在该发育阶段，萼片原基变得可见，B和C类花同源异型基因首先表达。AIL 6与ANT位点的子集结合，表明AIL 6调节与ANT相同的靶基因中的一些而不是全部。ANT和AIL 6结合位点与参与分生组织和花器官发育相关的许多生物学过程的基因相关。比较与ANT和AIL 6 ChIP-Seq峰相关的基因和ANT和/或AIL 6活性扰动后差异表达的基因，鉴定了ANT和AIL 6调节的可能直接靶标。这些基因包括B类和C类花同源异型基因、生长调节基因和参与维管发育的基因。
Arabidopsis ANT and AIL6 directly regulate genes involved in different aspects of early flower development including genes that specify floral organ identity and those that regulate growth and vascular development. Arabidopsis flower primordia give rise to organ primordia in stereotypical positions within four concentric whorls. Floral organ primordia in each whorl undergo distinct developmental programs to become one of four organ types (sepals, petals, stamens, and carpels). The Arabidopsis transcription factors AINTEGUMENTA (ANT) and AINTEGUMENTA-LIKE6 (AIL6) are required for correct positioning of floral organ initiation, contribute to the specification of floral organ identity, and regulate the growth and morphogenesis of developing floral organs. To gain insight into the molecular mechanisms by which ANT and AIL6 contribute to floral organogenesis, we identified the genome-wide binding sites of both ANT and AIL6 in stage 3 flower primordia, the developmental stage at which sepal primordia become visible and class B and C floral homeotic genes are first expressed. AIL6 binds to a subset of ANT sites, suggesting that AIL6 regulates some but not all of the same target genes as ANT. ANT- and AIL6-binding sites are associated with genes involved in many biological processes related to meristem and flower organ development. Comparison of genes associated with both ANT and AIL6 ChIP-Seq peaks and those differentially expressed after perturbation of ANT and/or AIL6 activity identified likely direct targets of ANT and AIL6 regulation. These include class B and C floral homeotic genes, growth regulatory genes, and genes involved in vascular development.
登录
查看更多内容
影响因子：
10.5
作者：
Dong H;Dumenil J;Lu FH;Na L;Vanhaeren H;Naumann C;Klecker M;Prior R;Smith C;McKenzie N;Saalbach G;Chen L;Xia T;Gonzalez N;Seguela M;Inze D;Dissmeyer N;Li Y;Bevan MW
通讯作者：
Bevan MW
DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
5.1
作者：
Han, Han;Krizek, Beth A.
通讯作者：
Krizek, Beth A.
影响因子：
7.2
作者：
Krizek, Beth A.;Blakley, Ivory C.;Loraine, Ann E.
通讯作者：
Loraine, Ann E.
影响因子：
64.5
作者：
JACK, T;BROCKMAN, LL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM