Label free detection of CD4+ and CD8+ T cells using the optofluidic ring resonator.
Label free detection of CD4+ and CD8+ T cells using the optofluidic ring resonator.
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DOI：
10.3390/s100605798
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Fan X
中科院分区：
文献类型：
--
作者：
Gohring JT;Fan X
We have demonstrated label free detection of CD4+ and CD8+ T-Lymphocyte whole cells and CD4+ T-Lymphocyte cell lysis using the optofluidic ring resonator (OFRR) sensor. The OFRR sensing platform incorporates microfluidics and photonics in a setup that utilizes small sample volume and achieves a fast detection time. In this work, white blood cells were isolated from healthy blood and the concentrations were adjusted to match T-Lymphocyte levels of individuals infected with HIV. Detection was accomplished by immobilizing CD4 and CD8 antibodies on the inner surface of the OFRR. Sensing results show excellent detection of CD4+ and CD8+ T-Lymphocyte cells at medically significant concentrations with a detection time of approximately 30 minutes. This work will lead to a rapid and low-cost sensing device that can provide a CD4 and CD8 count as a measure of HIV progression.
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影响因子：
3.8
作者：
Zhu, Hongying;White, Ian M.;Fan, Xudong
通讯作者：
Fan, Xudong
影响因子：
6.1
作者：
Wang, Yao-Nan;Kang, Yuejun;Li, Dongqing
通讯作者：
Li, Dongqing
影响因子：
3
作者：
Gazzard, B.
通讯作者：
Gazzard, B.
影响因子：
56.9
作者：
Autran, B;Carcelain, G;Leibowitch, J
通讯作者：
Leibowitch, J
影响因子：
15.9
作者：
ROEDERER, M;DUBS, JG;HERZENBERG, LA
通讯作者：
HERZENBERG, LA