Molecular Characterization and Functional Analysis of Two Petunia PhEILs.
Molecular Characterization and Functional Analysis of Two Petunia PhEILs.
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两种矮牵牛 PhEIL 的分子表征和功能分析。
DOI：
10.3389/fpls.2016.01606
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发表时间：
2016
影响因子：
5.6
通讯作者：
Yu Y
中科院分区：
文献类型：
--
作者：
Liu F;Hu L;Cai Y;Lin H;Liu J;Yu Y
乙烯在许多植物的花衰老过程中起着重要作用。拟南芥乙烯不敏感基因3（EIN 3）及其同源基因EIL 1是乙烯信号转导的下游元件。然而，EILs在花衰老过程中的功能仍不清楚。在这里，矮牵牛EIL基因PhEIL 2被分离出来。系统进化树分析表明，PhEIL 1和PhEIL 2分别与拟南芥AtEIL 3和AtEIL 1同源，PhEIL 1的编码基因已被分离。PhEIL 1和PhEIL 2在花冠中的表达量最高，并且在花冠衰老过程中表达量增加。乙烯处理增加了PhEIL 1的mRNA水平，但降低了PhEIL 2的mRNA水平。VIGS介导的PhEIL 1和PhEIL 2沉默延迟花衰老，并显着降低乙烯的产生和PhERF 3和PhCP 2，两个衰老相关基因在矮牵牛花的表达。PhEIL 2蛋白C端353-612个氨基酸的转录激活结构域被鉴定，酵母双杂交和双分子荧光互补实验表明PhEIL 2与PhEIL 1相互作用，表明PhEIL 1和PhEIL 2可能形成异二聚体来识别它们的靶标。矮牵牛PhEIL 1和PhEIL 2的分子特征与绿豆和拟南芥不同。
Ethylene plays an important role in flower senescence of many plants. Arabidopsis ETHYLENE INSENSITIVE3 (EIN3) and its homolog EIL1 are the downstream component of ethylene signaling transduction. However, the function of EILs during flower senescence remains unknown. Here, a petunia EIL gene, PhEIL2, was isolated. Phylogenetic tree showed that PhEIL1, whose coding gene is previously isolated, and PhEIL2 are the homologs of Arabidopsis AtEIL3 and AtEIL1, respectively. The expression of both PhEIL1 and PhEIL2 is the highest in corollas and increased during corolla senescence. Ethylene treatment increased the mRNA level of PhEIL1 but reduced that of PhEIL2. VIGS-mediated both PhEIL1 and PhEIL2 silencing delayed flower senescence, and significantly reduced ethylene production and the expression of PhERF3 and PhCP2, two senescence-associated genes in petunia flowers. The PhEIL2 protein activating transcription domain is identified in the 353-612-amino acids at C-terminal of PhEIL2 and yeast two-hybrid and bimolecular fluorescence complementation assays show that PhEIL2 interacts with PhEIL1, suggesting that PhEIL1 and PhEIL2 might form heterodimers to recognize their targets. These molecular characterizations of PhEIL1 and PhEIL2 in petunia are different with those of in Vigna radiata and Arabidopsis.
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影响因子：
56.9
作者：
Alonso, JM;Stepanova, AN;Ecker, JR
通讯作者：
Ecker, JR
影响因子：
6.9
作者：
Iordachescu, M;Verlinden, S
通讯作者：
Verlinden, S
影响因子：
7.4
作者：
Lee, JH;Kim, WT
通讯作者：
Kim, WT
DOI：
10.1126/science.1225974
发表时间：
2012-10-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Qiao H;Shen Z;Huang SS;Schmitz RJ;Urich MA;Briggs SP;Ecker JR
通讯作者：
Ecker JR
影响因子：
64.5
作者：
Potuschak, T;Lechner, E;Genschik, P
通讯作者：
Genschik, P