Nucleotide sequence polymorphism at the apical membrane antigen-1 locus reveals population history of Plasmodium vivax in Thailand.
Nucleotide sequence polymorphism at the apical membrane antigen-1 locus reveals population history of Plasmodium vivax in Thailand.
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DOI：
10.1016/j.meegid.2009.07.005
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发表时间：
2009-12
影响因子：
3.2
通讯作者：
Hughes, Austin L.
中科院分区：
文献类型：
--
作者：
Putaporntip, Chaturong;Jongwutiwes, Somchai;Grynberg, Priscila;Cui, Liwang;Hughes, Austin L.
顶膜抗原-1是用于包含在针对人类疟疾寄生虫间日疟原虫的疫苗中的候选物。我们收集了231个完整的序列，编码这种抗原（pvama-1）的基因，从泰国的三个地区，最广泛的收集，到目前为止，在这个位点的序列。结构域II环（先前作为潜在的疫苗组分提及）几乎完全保守，在单个序列中观察到单个氨基酸变体（I313 R）。基因的3′部分（结构域II至终止密码子）显示出比5′部分（起始密码子至结构域I）显著更低的核苷酸多样性;并且在具有多个结构域II序列的单倍型中可能发现给定的结构域I序列。这些结果暗示了结构域I和II之间的重组热点。我们发现泰国的三个地区（西北部，东部和南部），其中收集在2007年之间的显着的地理细分。自1990年以来，所有三个区域的间日疟原虫感染人数总体下降;但西北部最近出现下降，南部的感染人数自2000年以来出现反弹。与人口的历史，氨基酸序列多样性是最大的西北。南部地区的序列多样性是三个地区中最低的，有迹象表明，在经历了一个瓶颈后，该地区的人口已经从少数创始人扩大。
Apical membrane antigen-1 is a candidate for inclusion in a vaccine for the human malaria parasite Plasmodium vivax. We collected 231 complete sequences of the gene encoding this antigen (pvama-1) from three regions of Thailand, the most extensive collection to date of sequences at this locus. The domain II loop (previously mentioned as a potential vaccine component) was almost completely conserved, with a single amino acid variant (I313R) observed in a single sequence. The 3′ portion of the gene (domain II through the stop codon) showed significantly lower nucleotide diversity than the 5′ portion (start codon through domain I); and a given domain I sequence might be found in a haplotype with more than one domain II sequence. These results imply a hotspot of recombination between domains I and II. We found significant geographic subdivision among the three regions of Thailand (NW, East, and South) in which collections were made in 2007. Numbers of P. vivax infections have experienced overall declines since 1990 in all three regions; but the decline has been most recent in the NW, and there has been a rebound in numbers of infections in the South since 2000. Consistent with population history, amino acid sequence diversity was greatest in the NW. The South, which had by far the lowest sequence diversity of the three regions, showed signs of a population that has expanded from a small number of founders after a bottleneck.
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影响因子：
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作者：
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