Ginsenoside Rb1 attenuates intestinal ischemia reperfusion induced renal injury by activating Nrf2/ARE pathway.
Ginsenoside Rb1 attenuates intestinal ischemia reperfusion induced renal injury by activating Nrf2/ARE pathway.
复制标题
人参皂苷 Rb1 通过激活 Nrf2/ARE 通路减轻肠缺血再灌注引起的肾损伤
DOI：
10.3390/molecules17067195
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发表时间：
2012-06-12
期刊：
影响因子：
--
通讯作者：
Xia ZY
中科院分区：
文献类型：
--
作者：
Sun Q;Meng QT;Jiang Y;Xia ZY
肠缺血再灌注（IIR）是一种严重的临床疾病，与同时发生的多器官功能障碍有关。本研究旨在探讨人参皂苷Rb1对IIR诱导的小鼠肾损伤的影响。采用上级肠系膜动脉（SMA）阻断45 min，再灌注2 h的方法建立小鼠肠缺血再灌注模型。IIR引起的肾损伤主要表现为血清BUN、Cr、NGAL升高，肾组织MDA含量升高，SOD含量降低。再灌注前腹腔注射人参皂苷Rb1（30、60 mg/kg）可减轻肾损伤，其作用与血清BUN、Cr、NGAL降低，肾组织MDA含量降低，SOD含量升高有关。免疫组化和Western blot结果显示，Rb1能显著逆转IIR诱导的肾损伤，并上调肾组织核因子红细胞2相关因子2（Nrf2）和血红素加氧酶-1（HO-1）的表达。我们的数据表明，TRB1通过激活Nrf2/ARE通路减轻肠缺血再灌注引起的急性肾损伤。
Intestinal ischemia reperfusion (IIR) is a serious clinical condition associated with simultaneous multiple organ dysfunction. The aim of this study was to investigate the effects of ginsenoside Rb1 on IIR induced renal injury in mice. An intestinal ischemia reperfusion mouse model was established by superior mesenteric artery (SMA) occlusion for 45 min, followed by reperfusion for 2 h. IIR induced renal injury characterized by increase of BUN, Cr and NGAL in serum, MDA levels and decrease of SOD levels in the renal tissues. Ginsenoside Rb1 (30, 60 mg/kg) given intraperitoneally before reperfusion attennuated renal injury, which was associated with decrease of BUN, Cr and NGAL in serum, MDA levels and increase of SOD levels in the renal tissues. Furthermore, the immunohistochemistry and Western blot data showed that ginsenoside Rb1 dramatically reversed IIR induced renal injury, associated with upregulated nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) in renal tissues. Our data suggests that ginsenoside Rb1 attenuates acute renal injury induced by intestinal ischemia reperfusion by activating the Nrf2/ARE pathway.
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影响因子：
2.4
作者：
Nakao A;Kaczorowski DJ;Sugimoto R;Billiar TR;McCurry KR
通讯作者：
McCurry KR
DOI：
10.1016/s0167-4781(00)00238-4
发表时间：
2000-12-15
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
Chan, JY;Kwong, M
通讯作者：
Kwong, M
NRF2：氧化应激中的INRF2（KEAP1）信号传导。
影响因子：
7.4
作者：
Kaspar, James W.;Niture, Suryakant K.;Jaiswal, Anil K.
通讯作者：
Jaiswal, Anil K.
影响因子：
13.8
作者：
GUTTERIDGE, JMC;HALLIWELL, B
通讯作者：
HALLIWELL, B
影响因子：
6.2
作者：
MCWHINNIE, DL;THOMPSON, JF;MORRIS, PJ
通讯作者：
MORRIS, PJ