Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation.
Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation.
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DOI：
10.1186/s12864-021-08099-8
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发表时间：
2021-11-23
期刊：
影响因子：
4.4
通讯作者：
Comizzoli P
中科院分区：
文献类型：
--
作者：
Amelkina O;Silva AMD;Silva AR;Comizzoli P
睾丸组织发育的细胞和分子机制的基础知识对于更好地理解性腺生物学和对非生理条件的反应至关重要。本研究的目的是（1）分析家猫睾丸发育中的转录组动态和（2）表征年龄对组织玻璃化冷冻初始反应的影响。在玻璃化冷冻前后，对成年猫和幼年猫的组织进行组织学、DNA完整性和RNA测序分析。转录组学研究结果能够进一步表征幼年期，区分早期和晚期幼年组织。基因表达和功能途径的变化是广泛的，从早期到晚期的青少年到成人的发展阶段。此外，与成年动物相比，幼龄动物的组织对玻璃化更具弹性，早期幼龄动物样品对玻璃化的反应最小，晚期幼龄动物样品的反应最接近成年组织。这是第一项研究报告了根据年龄和暴露于冷冻保存的猫睾丸的转录组动态结合结构分析的综合数据集。它提供了一个全面的网络功能的条款和途径，受年龄的影响，在家猫和丰富的成年或幼年睾丸组织。在线版本包含补充材料，可通过10.1186/s12864-021-08099-8获得。
Fundamental knowledge of cellular and molecular mechanisms in developing testicular tissues is critical to better understand gonadal biology and responses to non-physiological conditions. The objective of our study was to (1) analyze transcriptome dynamics in developing testis of the domestic cat and (2) characterize age effects on the initial response of the tissue to vitrification. Tissues from adult and juvenile cats were processed for histology, DNA integrity, and RNA sequencing analyses before and after vitrification. Transcriptomic findings enabled to further characterize juvenile period, distinguishing between early and late juvenile tissues. Changes in gene expression and functional pathways were extensive from early to late juvenile to adult development stages. Additionally, tissues from juvenile animals were more resilient to vitrification compared to adult counterparts, with early juvenile sample responding the least to vitrification and late juvenile sample response being closest to adult tissues. This is the first study reporting comprehensive datasets on transcriptomic dynamic coupled with structural analysis of the cat testis according to the age and exposure to cryopreservation. It provides a comprehensive network of functional terms and pathways that are affected by age in the domestic cat and are either enriched in adult or juvenile testicular tissues. The online version contains supplementary material available at 10.1186/s12864-021-08099-8.
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影响因子：
3.6
作者：
França, LR;Godinho, CL
通讯作者：
Godinho, CL
影响因子：
44.1
作者：
Guo J;Grow EJ;Mlcochova H;Maher GJ;Lindskog C;Nie X;Guo Y;Takei Y;Yun J;Cai L;Kim R;Carrell DT;Goriely A;Hotaling JM;Cairns BR
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Cairns BR
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3.6
作者：
Griswold MD
通讯作者：
Griswold MD
影响因子：
10.5
作者：
Cabili, Moran N.;Trapnell, Cole;Rinn, John L.
通讯作者：
Rinn, John L.
通过转录组和小 RNA 测序探索新鲜和冻融公猪精子中的 miRNA 和 mRNA 谱。
影响因子：
5.6
作者：
Dai, Ding-Hui;Qazi, Izhar Hyder;Zeng, Chang-Jun
通讯作者：
Zeng, Chang-Jun