Nitric Oxide Regulates Seedling Growth and Mitochondrial Responses in Aged Oat Seeds.
Nitric Oxide Regulates Seedling Growth and Mitochondrial Responses in Aged Oat Seeds.
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一氧化氮调节老化燕麦种子中的幼苗生长和线粒体反应
DOI：
10.3390/ijms19041052
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发表时间：
2018-04-02
影响因子：
5.6
通讯作者：
Mao P
中科院分区：
文献类型：
--
作者：
Mao C;Zhu Y;Cheng H;Yan H;Zhao L;Tang J;Ma X;Mao P
线粒体是植物细胞中活性氧（ROS）的来源，在线粒体电子传递链（ETC）和三羧酸循环（TCA）中起着重要作用，但其对种子萌发、幼苗生长以及线粒体对非生物胁迫的响应的调控机制尚不清楚。本研究以燕麦（Avena sativa L.）种子为材料，研究了种子萌发、胚线粒体抗氧化反应及老化过程中蛋白质谱的变化。暴露于外源性一氧化氮（NO）治疗。结果表明，老化种子线粒体中H2 O2的积累显著增加。人工老化可导致种子活力丧失，表现为种子发芽率下降和平均发芽时间延长。幼苗生长也受到抑制。一些酶，包括过氧化氢酶（CAT），谷胱甘肽还原酶（GR），脱氢抗坏血酸还原酶（达尔），和单脱氢抗坏血酸还原酶（MDHAR），保持在抗坏血酸-谷胱甘肽（AsA-GSH）清除系统的较低水平。蛋白质组学分析显示，与TCA循环相关的一些蛋白质表达下调，与线粒体ETC相关的几种酶表达上调。在老化燕麦种子中应用0.05 mM NO，观察到保护作用，证明了种子活力的改善和线粒体中H2 O2清除能力的增加。AsA-GSH清除系统中CAT、GR、MDHAR和达尔的活性也较高，TCA循环相关酶（苹果酸脱氢酶、琥珀酸CoA连接酶、富马酸水合酶）增强，细胞色素途径被抑制时，交替途径被激活。因此，我们的研究结果表明，幼苗生长和种子发芽率可以保持一定的水平，在老化的燕麦种子，主要取决于较低的TCA循环和AsA-GSH的NO调节。因此，可以得出结论，在老化的燕麦种子中应用0.05 mM NO通过增强线粒体TCA循环和激活替代途径来改善种子活力。
Mitochondria are the source of reactive oxygen species (ROS) in plant cells and play a central role in the mitochondrial electron transport chain (ETC) and tricarboxylic acid cycle (TCA) cycles; however, ROS production and regulation for seed germination, seedling growth, as well as mitochondrial responses to abiotic stress, are not clear. This study was conducted to obtain basic information on seed germination, embryo mitochondrial antioxidant responses, and protein profile changes in artificial aging in oat seeds (Avena sativa L.) exposed to exogenous nitric oxide (NO) treatment. The results showed that the accumulation of H2O2 in mitochondria increased significantly in aged seeds. Artificial aging can lead to a loss of seed vigor, which was shown by a decline in seed germination and the extension of mean germination time (MGT). Seedling growth was also inhibited. Some enzymes, including catalase (CAT), glutathione reductase (GR), dehydroascorbate reductase (DHAR), and monodehydroascorbate reductase (MDHAR), maintained a lower level in the ascorbate-glutathione (AsA-GSH) scavenging system. Proteomic analysis revealed that the expression of some proteins related to the TCA cycle were down-regulated and several enzymes related to mitochondrial ETC were up-regulated. With the application of 0.05 mM NO in aged oat seeds, a protective effect was observed, demonstrated by an improvement in seed vigor and increased H2O2 scavenging ability in mitochondria. There were also higher activities of CAT, GR, MDHAR, and DHAR in the AsA-GSH scavenging system, enhanced TCA cycle-related enzymes (malate dehydrogenase, succinate-CoA ligase, fumarate hydratase), and activated alternative pathways, as the cytochrome pathway was inhibited. Therefore, our results indicated that seedling growth and seed germinability could retain a certain level in aged oat seeds, predominantly depending on the lower NO regulation of the TCA cycle and AsA-GSH. Thus, it could be concluded that the application of 0.05 mM NO in aged oat seeds improved seed vigor by enhancing the mitochondrial TCA cycle and activating alternative pathways for improvement.
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影响因子：
4.8
作者：
Johnson, JD;Mehus, JG;Lambeth, DO
通讯作者：
Lambeth, DO
DOI：
10.1073/pnas.96.14.8271
发表时间：
1999-07-06
影响因子：
11.1
作者：
Maxwell, DP;Wang, Y;McIntosh, L
通讯作者：
McIntosh, L
影响因子：
4.3
作者：
Huang, X;von Rad, U;Durner, J
通讯作者：
Durner, J
影响因子：
3.5
作者：
MILLAR, AH;WISKICH, JT;DAY, DA
通讯作者：
DAY, DA
影响因子：
9.4
作者：
Jardim-Messeder, Douglas;Caverzan, Andreia;Galina, Antonio
通讯作者：
Galina, Antonio