Thyroid Receptor Antagonism of Chemicals Extracted from Personal Silicone Wristbands within a Papillary Thyroid Cancer Pilot Study.
Thyroid Receptor Antagonism of Chemicals Extracted from Personal Silicone Wristbands within a Papillary Thyroid Cancer Pilot Study.
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DOI：
10.1021/acs.est.0c05972
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发表时间：
2020-12-01
影响因子：
11.4
通讯作者：
Stapleton HM
中科院分区：
文献类型：
--
作者：
Kassotis CD;Herkert NJ;Hammel SC;Hoffman K;Xia Q;Kullman SW;Sosa JA;Stapleton HM
研究表明，甲状腺癌的发病率正在上升，环境暴露被认为是其中的一个因素。为了探索这种可能性，我们进行了一项初步研究，以调查从甲状腺癌队列中的个人硅胶腕带采样器中分离出来的化学混合物对甲状腺的破坏生物活性。具体地说，我们评估了从北卡罗来纳州中部参加乳头状甲状腺癌病例对照研究的成年人佩戴的腕带(n=72)中提取的化学混合物的TRβ拮抗作用。部分腕带被溶剂提取并通过质谱仪进行分析，以对一组半挥发性化学物质进行定量。每条腕带的第二个提取物用于生物测定，以定量人胚胎肾细胞(HEK293/17)的TRβ拮抗作用，浓度范围为原始提取物的0.1%至10%(按体积计)。在1%的提取物浓度下，大约70%的样品提取物表现出显著的TR-β拮抗作用，平均为30%，范围为0-100%。在测试浓度为5%和10%的样品中，有20%的样品被抑制了细胞活力。拮抗作用与几种邻苯二甲酸酯、有机磷酸酯和溴化阻燃剂的腕带浓度呈正相关。这些结果表明，个人被动采样器可能有助于评估人们日常接触的混合物的生物活性。我们还报告了甲状腺受体拮抗、化学浓度和乳头状甲状腺癌病例状态之间的初步关联。未来的研究应该利用更大的样本量、前瞻性的数据收集和血清甲状腺激素水平的测量(这在本研究中是不可能的)来更全面地评估这些相关性。
Research suggests that thyroid cancer incidence rates are increasing, and environmental exposures have been postulated to be playing a role. To explore this possibility, we conducted a pilot study to investigate the thyroid disrupting bioactivity of chemical mixtures isolated from personal silicone wristband samplers within a thyroid cancer cohort. Specifically, we evaluated TRβ antagonism of chemical mixtures extracted from wristbands (n = 72) worn by adults in central North Carolina participating in a case-control study on papillary thyroid cancer. Sections of wristbands were solvent-extracted and analyzed via mass spectrometry to quantify a suite of semivolatile chemicals. A second extract from each wristband was used in a bioassay to quantify TRβ antagonism in human embryonic kidney cells (HEK293/17) at concentrations ranging from 0.1 to 10% of the original extract (by volume). Approximately 70% of the sample extracts tested at a 1% extract concentration exhibited significant TRβ antagonism, with a mean of 30% and a range of 0–100%. Inhibited cell viability was noted in >20% of samples that were tested at 5 and 10% concentrations. Antagonism was positively associated with wristband concentrations of several phthalates, organophosphate esters, and brominated flame retardants. These results suggest that personal passive samplers may be useful in evaluating the bioactivities of mixtures that people contact on a daily basis. We also report tentative associations between thyroid receptor antagonism, chemical concentrations, and papillary thyroid cancer case status. Future research utilizing larger sample sizes, prospective data collection, and measurement of serum thyroid hormone levels (which were not possible in this study) should be utilized to more comprehensively evaluate these associations.
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DOI：
10.3390/ijerph14040355
发表时间：
2017-03-29
影响因子：
--
作者：
Benedetti M;Zona A;Beccaloni E;Carere M;Comba P
通讯作者：
Comba P
影响因子：
4.3
作者：
Dixon HM;Scott RP;Holmes D;Calero L;Kincl LD;Waters KM;Camann DE;Calafat AM;Herbstman JB;Anderson KA
通讯作者：
Anderson KA
DOI：
10.1016/j.jsbmb.2008.06.010
发表时间：
2008-09-01
影响因子：
4.1
作者：
Bryzgalova, Galina;Effendic, Suad;Grover, Gary J.
通讯作者：
Grover, Gary J.
影响因子：
--
作者：
Crofton, Kevin M.
通讯作者：
Crofton, Kevin M.
影响因子：
8.3
作者：
Arrebola, Juan P.;Pumarega, Jose;Olea, Nicolas
通讯作者：
Olea, Nicolas