MSCs Become Collagen-Type I Producing Cells with Different Phenotype in Allogeneic and Syngeneic Bone Marrow Transplantation.
MSCs Become Collagen-Type I Producing Cells with Different Phenotype in Allogeneic and Syngeneic Bone Marrow Transplantation.
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DOI：
10.3390/ijms22094895
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发表时间：
2021-05-05
影响因子：
5.6
通讯作者：
Shimmura S
中科院分区：
文献类型：
--
作者：
Rusch RM;Ogawa Y;Sato S;Morikawa S;Inagaki E;Shimizu E;Tsubota K;Shimmura S
间充质干细胞（MSC）已广泛用于治疗应用了几十年。然而，越来越多的证据表明，诸如起源部位和植入前处理等因素对结果有着至关重要的影响。本研究探讨新鲜分离的间充质干细胞在异体移植后泪腺中的作用。为此目的，从转基因GFP小鼠中分离MSC并移植到同种异体和同基因受体中。同基因间充质干细胞保持球形，而同种异体间充质干细胞作为间质基质中的梭形细胞移植到组织中。此外，在两种模型的受体中，MSC在超过85%至95%的检测到的GFP+ MSC中产生I型胶原蛋白，与同基因模型相比，推测其导致同种异体受体中的致病性纤维化。这些发现表明同种异体MSC的行为与同基因MSC完全不同，突出了理解MSC背后确切机制的重要性。
Mesenchymal stem cells (MSCs) have been widely used in therapeutic applications for many decades. However, more and more evidence suggests that factors such as the site of origin and pre-implantation treatment have a crucial impact on the result. This study investigates the role of freshly isolated MSCs in the lacrimal gland after allogeneic transplantation. For this purpose, MSCs from transgenic GFP mice were isolated and transplanted into allogeneic and syngeneic recipients. While the syngeneic MSCs maintained a spherical shape, allogeneic MSCs engrafted into the tissue as spindle-shaped cells in the interstitial stroma. Furthermore, the MSCs produced collagen type I in more than 85% to 95% of the detected GFP+ MSCs in the recipients of both models, supposedly contributing to pathogenic fibrosis in allogeneic recipients compared to syngeneic models. These findings indicate that allogeneic MSCs act completely differently from syngeneic MSCs, highlighting the importance of understanding the exact mechanisms behind MSCs.
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小鼠骨髓中多能间充质干细胞的前瞻性鉴定、分离和系统移植。
DOI：
10.1084/jem.20091046
发表时间：
2009-10-26
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Morikawa S;Mabuchi Y;Kubota Y;Nagai Y;Niibe K;Hiratsu E;Suzuki S;Miyauchi-Hara C;Nagoshi N;Sunabori T;Shimmura S;Miyawaki A;Nakagawa T;Suda T;Okano H;Matsuzaki Y
通讯作者：
Matsuzaki Y
影响因子：
7.5
作者：
Duffy MM;Ritter T;Ceredig R;Griffin MD
通讯作者：
Griffin MD
影响因子：
7.7
作者：
Ogawa Y;Morikawa S;Okano H;Mabuchi Y;Suzuki S;Yaguchi T;Sato Y;Mukai S;Yaguchi S;Inaba T;Okamoto S;Kawakami Y;Tsubota K;Matsuzaki Y;Shimmura S
通讯作者：
Shimmura S
影响因子：
5.2
作者：
in't Anker, PS;Scherjon, SA;Kanhai, HHH
通讯作者：
Kanhai, HHH
影响因子：
6.2
作者：
FRIEDENSTEIN, AJ;CHAILAKHYAN, RK;KEILISSB.IV
通讯作者：
KEILISSB.IV