Afferent nerve activity in a mouse model increases with faster bladder filling rates in vitro, but voiding behavior remains unaltered in vivo.
Afferent nerve activity in a mouse model increases with faster bladder filling rates in vitro, but voiding behavior remains unaltered in vivo.
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DOI：
10.1152/ajpregu.00156.2022
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发表时间：
2022-11-01
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影响因子：
--
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膀胱中的储存和空隙功能是众所周知的，但是协调这些行为的基本生理事件仍然难以捉摸。我们试图了解空隙功能如何受膀胱填充速率的影响。我们假设更快的填充率将增加传入的感觉活动并增加排尿率。在体内，这将意味着在较小的空隙体积中，经历更快的膀胱填充的动物会更频繁地空隙。为了检验这一假设，我们使用离体小鼠膀胱制剂在不同的填充速率过程中测量了传入的神经活性，并在正常行为的小鼠中评估了空隙频率（尿素）。膀胱传入神经活动取决于填充率，在给定音量下，填充的传入神经活动的增加。在尿反射笼中测量了体内空隙行为。雄性和雌性小鼠可以进入自来水，或者诱导含有5％蔗糖的水，以诱导更快的膀胱填充率。食用5％蔗糖的小鼠中液体摄入量急剧增加。不出所料，蔗糖组的排尿频率升高。但是，即使尿液产生的速度大大提高，两种性别的空隙量也保持不变。尽管更快的填充率会产生更高的传入神经率，但在体内这并没有转化为更频繁的较小体积的空隙。这表明传入神经活动只是导致从膀胱填充到排尿的转换的一个因素。与传入的神经活动一起，中枢神经系统和唤醒状态的较高中心对于协调排尿反射至关重要。
Storage and voiding functions in urinary bladder are well-known, yet fundamental physiological events coordinating these behaviors remain elusive. We sought to understand how voiding function is influenced by the rate at which the bladder fills. We hypothesized that faster filling rates would increase afferent sensory activity and increase micturition rate. In vivo, this would mean animals experiencing faster bladder filling would void more frequently with smaller void volumes. To test this hypothesis, we measured afferent nerve activity during different filling rates using an ex vivo mouse bladder preparation and assessed voiding frequency in normally behaving mice noninvasively (UroVoid). Bladder afferent nerve activity depended on the filling rate, with faster filling increasing afferent nerve activity at a given volume. Voiding behavior in vivo was measured in UroVoid cages. Male and female mice were given access to tap water or, to induce faster bladder filling rates, water containing 5% sucrose. Fluid intake increased dramatically in mice consuming 5% sucrose. As expected, micturition frequency was elevated in the sucrose group. However, even with the greatly increased rate of urine production, void volumes were unchanged in both genders. Although faster filling rates generated higher afferent nerve rates ex vivo, this did not translate into more frequent, smaller-volume voids in vivo. This suggests afferent nerve activity is only one factor contributing to the switch from bladder filling to micturition. Together with afferent nerve activity, higher centers in the central nervous system and the state of arousal are likely critical to coordinating the micturition reflex.
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影响因子：
4.5
作者：
Drake MJ;Kanai A;Bijos DA;Ikeda Y;Zabbarova I;Vahabi B;Fry CH
通讯作者：
Fry CH
影响因子：
4.6
作者：
Mawla I;Schrepf A;Ichesco E;Harte SE;Klumpp DJ;Griffith JW;Strachan E;Yang CC;Lai H;Andriole G;Magnotta VA;Kreder K;Clauw DJ;Harris RE;Clemens JQ;Landis JR;Mullins C;Rodriguez LV;Mayer EA;Kutch JJ
通讯作者：
Kutch JJ
影响因子：
5.6
作者：
Parsons BA;Drake MJ;Gammie A;Fry CH;Vahabi B
通讯作者：
Vahabi B
影响因子：
6.6
作者：
DORR, W
通讯作者：
DORR, W
影响因子：
5.5
作者：
Rong, W;Spyer, KM;Burnstock, G
通讯作者：
Burnstock, G