Identification of a key locus, qNL3.1, associated with seed germination under salt stress via a genome-wide association study in rice.
Identification of a key locus, qNL3.1, associated with seed germination under salt stress via a genome-wide association study in rice.
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通过全基因组关联研究鉴定与盐胁迫下水稻种子萌发相关的关键位点qNL3.1
DOI：
10.1007/s00122-023-04252-x
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发表时间：
2023-03-13
影响因子：
5.4
通讯作者：
Cheng, Jinping
中科院分区：
文献类型：
--
作者：
Zhan, Chengfang;Zhu, Peiwen;Chen, Yongji;Chen, Xinyi;Liu, Kexin;Chen, Shanshan;Hu, Jiaxiao;He, Ying;Xie, Ting;Luo, Shasha;Yang, Zeyuan;Chen, Sunlu;Tang, Haijuan;Zhang, Hongsheng;Cheng, Jinping
通过全基因组关联研究，确定了与盐胁迫下水稻种子萌发显著相关的关键基因qNL3.1上的两个致病基因OsTTL和OsSAPK1，它们可以促进水稻种子在盐胁迫下的萌发。水稻是一种对盐分敏感的作物，其种子萌发决定了随后的成苗和产量。以种子发芽率(GR)、发芽指数(GI)、达到50%发芽的时间(T50)和平均发芽水平(ML)为指标，对168份盐胁迫下种子萌发的遗传控制进行了研究。在盐胁迫下，不同材料的种子萌发存在着广泛的自然变异。相关分析表明，盐胁迫下种子萌发过程中GR、GI与ML呈显著正相关，与T50呈显著负相关。共鉴定出49个与盐胁迫下种子萌发显著相关的基因座，其中7个在两年中都被鉴定出来。通过比较，有16个座位与以前的QTL定位在一起，其余33个座位可能是新的。与qLTG-3共定位的qNL3.1可能是盐胁迫下种子萌发的关键基因。对候选基因的分析表明，与反式甲状腺激素样蛋白OsTTL和丝氨酸/苏氨酸蛋白激酶OsSAPK1相似的两个基因是qNL3.1的致病基因。发芽试验表明，与野生型相比，Ostt1和Ossak1突变体都显著降低了盐胁迫下种子的发芽率。单倍型分析表明，OsTTL的Hap1和OsSAPK1基因的Hap1是优良的等位基因，它们的结合导致了种子在盐胁迫下的高萌发。筛选出了8份盐胁迫下种子萌发表现优异的材料，它们可以提高水稻种子在盐胁迫下的萌发率。网上版载有补充材料，可在10.1007/s00122-023-04252-x查阅。
Two causal OsTTL and OsSAPK1 genes of the key locus qNL3.1 significantly associated with seed germination under salt stress were identified via a genome-wide association study, which could improve rice seed germination under salt stress. Rice is a salt-sensitive crop, and its seed germination determines subsequent seedling establishment and yields. In this study, 168 accessions were investigated for the genetic control of seed germination under salt stress based on the germination rate (GR), germination index (GI), time at which 50% germination was achieved (T50) and mean level (ML). Extensive natural variation in seed germination was observed among accessions under salt stress. Correlation analysis showed significantly positive correlations among GR, GI and ML and a negative correlation with T50 during seed germination under salt stress. Forty-nine loci significantly associated with seed germination under salt stress were identified, and seven of these were identified in both years. By comparison, 16 loci were colocated with the previous QTLs, and the remaining 33 loci might be novel. qNL3.1, colocated with qLTG-3, was simultaneously identified with the four indices in two years and might be a key locus for seed germination under salt stress. Analysis of candidate genes showed that two genes, the similar to transthyretin-like protein OsTTL and the serine/threonine protein kinase OsSAPK1, were the causal genes of qNL3.1. Germination tests indicated that both Osttl and Ossapk1 mutants significantly reduced seed germination under salt stress compared to the wild type. Haplotype analysis showed that Hap.1 of OsTTL and Hap.1 of OsSAPK1 genes were excellent alleles, and their combination resulted in high seed germination under salt stress. Eight accessions with elite performance of seed germination under salt stress were identified, which could improve rice seed germination under salt stress. The online version contains supplementary material available at 10.1007/s00122-023-04252-x.
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影响因子：
16.6
作者：
Crowell S;Korniliev P;Falcão A;Ismail A;Gregorio G;Mezey J;McCouch S
通讯作者：
McCouch S
DOI：
10.1186/s12284-018-0244-z
发表时间：
2018-09-10
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Chen HC;Cheng WH;Hong CY;Chang YS;Chang MC
通讯作者：
Chang MC
影响因子：
7.2
作者：
Joosen, Ronny V. L.;Kodde, Jan;Hilhorst, Henk W. M.
通讯作者：
Hilhorst, Henk W. M.
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.
拮抗受体的表观遗传调控赋予稻瘟病抗性和产量平衡。
影响因子：
56.9
作者：
Deng, Yiwen;Zhai, Keran;He, Zuhua
通讯作者：
He, Zuhua