The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively.
The transcription factor OsbHLH035 mediates seed germination and enables seedling recovery from salt stress through ABA-dependent and ABA-independent pathways, respectively.
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DOI：
10.1186/s12284-018-0244-z
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发表时间：
2018-09-10
期刊：
影响因子：
--
通讯作者：
Chang MC
中科院分区：
文献类型：
--
作者：
Chen HC;Cheng WH;Hong CY;Chang YS;Chang MC
许多转录因子（transcription factors，TF），如碱性螺旋-环-螺旋（basic helix-loop-helix，bHLH）家族中的转录因子，在调节植物生长和植物对非生物胁迫的反应中起着重要作用。OsbHLH 035的表达受干旱和盐胁迫的诱导。然而，其在水稻生长发育和盐响应中的功能作用仍不清楚。bHLH TF OsbHLH 035是一个盐诱导基因，主要在萌发的种子和幼苗中表达。GFP融合蛋白OsbHLH 035在水稻转基因植株中的稳定表达表明，该蛋白主要定位于细胞核。Osbhlh 035突变体表现出延迟的种子萌发，特别是在盐胁迫条件下。与此同时，Osbhlh 035突变体种子中脱落酸（阿坝）含量过量积累，阿坝生物合成基因OsABA 2和OsAAO 3的表达上调;此外，与野生型（WT）种子相比，盐诱导的阿坝分解代谢基因OsABA 8 ox 1的表达下调。此外，Osbhlh 035突变体幼苗不能从盐胁迫处理中恢复。盐处理后Osbhlh 035幼苗地上部组织中钠积累过量，而地上部组织中钠含量略有降低。此外，钠转运蛋白OsHKT 1;3和1;5的表达分别在Osbhlh 035气生和陆生组织中减少。此外，遗传互补可以恢复延迟的种子萌发和盐处理的Osbhlh 035幼苗受损恢复正常生长。OsbHLH 035分别通过ABA依赖性和ABA非依赖性激活OsHKT途径介导盐胁迫缓解后的种子萌发和幼苗恢复。本文的在线版本（10.1186/s12284-018-0244-z）包含补充材料，可供授权用户使用。
Many transcription factors (TFs), such as those in the basic helix-loop-helix (bHLH) family, are important for regulating plant growth and plant responses to abiotic stress. The expression of OsbHLH035 is induced by drought and salinity. However, its functional role in rice growth, development, and the salt response is still unknown. The bHLH TF OsbHLH035 is a salt-induced gene that is primarily expressed in germinating seeds and seedlings. Stable expression of GFP-fused OsbHLH035 in rice transgenic plants revealed that this protein is predominantly localized to the nucleus. Osbhlh035 mutants show delayed seed germination, particularly under salt-stress conditions. In parallel, abscisic acid (ABA) contents are over-accumulated, and the expression of the ABA biosynthetic genes OsABA2 and OsAAO3 is upregulated; furthermore, compared with that in wild-type (WT) seedlings, the salt-induced expression of OsABA8ox1, an ABA catabolic gene, in germinating Osbhlh035 mutant seeds is downregulated. Moreover, Osbhlh035 mutant seedlings are unable to recover from salt-stress treatment. Consistently, sodium is over-accumulated in aerial tissues but slightly reduced in terrestrial tissues from Osbhlh035 seedlings after salt treatment. Additionally, the expression of the sodium transporters OsHKT1;3 and 1;5 is reduced in Osbhlh035 aerial and terrestrial tissues, respectively. Furthermore, genetic complementation can restore both the delayed seed germination and the impaired recovery of salt-treated Osbhlh035 seedlings to normal growth. OsbHLH035 mediates seed germination and seedling recovery after salt stress relief through the ABA-dependent and ABA-independent activation of OsHKT pathways, respectively. The online version of this article (10.1186/s12284-018-0244-z) contains supplementary material, which is available to authorized users.
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DOI：
10.1093/dnares/dst012
发表时间：
2013-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Behnam B;Iuchi S;Fujita M;Fujita Y;Takasaki H;Osakabe Y;Yamaguchi-Shinozaki K;Kobayashi M;Shinozaki K
通讯作者：
Shinozaki K
影响因子：
3.7
作者：
Heang D;Sassa H
通讯作者：
Sassa H
影响因子：
11.4
作者：
Kushiro, T;Okamoto, M;Nambara, E
通讯作者：
Nambara, E
影响因子：
11.6
作者：
Fukao, Takeshi;Yeung, Elaine;Bailey-Serres, Julia
通讯作者：
Bailey-Serres, Julia
影响因子：
11.6
作者：
GIRAUDAT, J;HAUGE, BM;GOODMAN, HM
通讯作者：
GOODMAN, HM