Pharmacokinetic Analysis of Carnosic Acid and Carnosol in Standardized Rosemary Extract and the Effect on the Disease Activity Index of DSS-Induced Colitis.
Pharmacokinetic Analysis of Carnosic Acid and Carnosol in Standardized Rosemary Extract and the Effect on the Disease Activity Index of DSS-Induced Colitis.
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DOI：
10.3390/nu13030773
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发表时间：
2021-02-27
期刊：
影响因子：
5.9
通讯作者：
Johnson JJ
中科院分区：
文献类型：
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作者：
Veenstra JP;Vemu B;Tocmo R;Nauman MC;Johnson JJ
迷迭香提取物(RE)是欧盟批准的一种食品防腐剂，含有有益于胃肠道健康的饮食植物化学物质。本研究探讨了稀土对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎的影响，并测定了小鼠灌胃饲料中植物化学物质的药代动力学。采用LC-MS/MS联用技术对迷迭香提取物中的单体成分进行了分离和鉴定，并测定了两种稀土二萜化合物鼠尾草酸(CA)和鼠尾草醇(CL)在小鼠体内的药代动力学。然后，观察稀土对DSS诱导的小鼠结肠炎疾病活动指数的影响。研究确定，与阴性对照相比，100 mg/kg稀土显著改善DSS诱导的结肠炎患者的DAI。随着DSS暴露的增加，Sestrin 2蛋白的表达在RE处理后降低。通过给予异硫氰酸荧光素(FITC)-葡聚糖和紧密连接蛋白ZO-1的Western印迹，肠屏障完整性也得到了改善。迷迭香提取物100 mg/kg能改善DSS诱导的小鼠结肠炎DAI，改善肠屏障完整性。本研究提示，稀土是一种有效的IBD预防药物。
Rosemary extract (RE) is an approved food preservative in the European Union and contains dietary phytochemicals that are beneficial for gastrointestinal health. This study investigated the effects of RE on dextran sodium sulfate (DSS)-induced colitis and also determined the pharmacokinetics of dietary phytochemicals administered to mice via oral gavage. Individual components of rosemary extract were separated and identified by LC–MS/MS. The pharmacokinetics of two major diterpenes from RE, carnosic acid (CA) and carnosol (CL), administered to mice via oral gavage were determined. Then, the effect of RE pre-treatment on the disease activity index (DAI) of DSS-induced colitis in mice was investigated. The study determined that 100 mg/kg RE significantly improved DAI in DSS-induced colitis compared to negative control. Sestrin 2 protein expression, which increased with DSS exposure, was reduced with RE treatment. Intestinal barrier integrity was also shown to improve via fluorescein isothiocyanate (FITC)–dextran administration and Western blot of zonula occludens-1 (ZO-1), a tight junction protein. Rosemary extract was able to improve the DAI of DSS-induced colitis in mice at a daily dose of 100 mg/kg and showed improvement in the intestinal barrier integrity. This study suggests that RE can be an effective preventative agent against IBD.
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影响因子：
9.7
作者：
Johnson, Jeremy J.
通讯作者：
Johnson, Jeremy J.
影响因子：
4.7
作者：
Alsamri, Halima;El Hasasna, Hussain;Iratni, Rabah
通讯作者：
Iratni, Rabah
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1.8
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LOLIGERS, J
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4.3
作者：
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通讯作者：
Al-Hassi, Hafid Omar
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