Immunodetection and localization of protein(s) related to retinal S‐antigen (arrestin) in kidney
Immunodetection and localization of protein(s) related to retinal S‐antigen (arrestin) in kidney
复制标题
肾脏中视网膜 S 抗原（抑制蛋白）相关蛋白的免疫检测和定位
DOI：
10.1016/0248-4900(92)90210-r
复制
发表时间：
1992
影响因子：
2.7
通讯作者：
J. Faure
中科院分区：
文献类型：
--
作者：
M. Mirshahi;Ahmad Razaghi;A. Vandewalle;F. Cluzeaud;Mohammed Tarraf;J. Faure
S抗原(Arrestin)是一种胞浆蛋白，调节视网膜视杆细胞的光信号转导。用抗视网膜S抗原的多克隆抗体，经凝胶过滤或免疫亲和层析，从牛肾可溶性提取物中鉴定出一种与S抗原免疫相关的蛋白质。在免疫印迹上，该蛋白被一组针对不同S抗原表位的单抗(单抗S2D2、S1A3和S9E2)识别，其表观分子量(48 KDa)与视网膜S抗原相同。用间接免疫细胞化学技术检测，3株单抗在大鼠肾切片上均呈特异性免疫反应。这三种单抗识别部分但不是全部肾小球细胞，通过使用单抗S9E2的免疫电子显微镜鉴定为上皮细胞。单抗S2D2和S1A3在所有肾小管上皮细胞均呈弥漫性胞浆染色。近端小管细胞呈弱免疫反应，远端和集合管细胞呈强阳性。相反，单抗S9E2的免疫反应仅限于免疫电子显微镜鉴定的远端小管和集合管中与嵌入细胞相对应的一个细胞亚群。利用单抗S9E2，近端小管细胞的标记定位于细胞质的顶端。这些结果表明，肾脏中存在两种或两种以上与视网膜S抗原免疫交叉反应的48 kDa蛋白。观察到的分布模式符合这样的假设，即这些蛋白可能在调节肾小球和肾小管上皮细胞中存在的G蛋白相关受体中发挥作用。
S‐antigen (arrestin) is a cytosolic protein which regulates phototransduction in retinal rods. A protein immunologically related to S‐antigen was identified in fractions from soluble extract of bovine kidney enriched by gel filtration or by immunoaffinity chromatography using a polyclonal antibody to retinal S‐antigen. On immunoblots, this protein was recognized by a panel of monoclonal antibodies (mAbs S2D2, S1A3 and S9E2) directed against different S‐antigen epitopes and displayed the same apparent molecular mass (48 kDa) as retinal S‐antigen. All three mAbs revealed a specific immunoreactivity by indirect immunocytochemical technique on rat kidney sections. The three mAbs recognized some but not all glomerular cells, identified as epithelial cells by immunoelectron microscopy using the mAb S9E2. Both mAbs S2D2 and S1A3 gave a diffuse cytoplasmic staining in all tubule cells. Proximal tubule cells exhibited a weak immunoreactivity, whereas distal and collecting tubule cells were strongly labeled. In contrast, the mAb S9E2 immunoreaction was restricted to a cell subpopulation from distal and collecting tubules corresponding to intercalated cells identified by immunoelectron microscopy. With the mAb S9E2, the labeling of proximal tubule cells was localized in the apical region of the cytoplasm. These results suggest that two or more 48‐kDa proteins immunologically cross‐reactive with retinal S‐antigen are present in kidney. The observed pattern of distribution is in keeping with the hypothesis that such proteins could play a role in the regulation of G‐protein‐related receptors present in renal glomerules and tubule epithelial cells.
登录
查看更多内容
大鼠肾集合管中的三种不同的细胞群。
DOI：
10.1152/ajpcell.1987.253.2.c323
发表时间：
1987
期刊：
The American journal of physiology
影响因子：
--
作者：
Holthöfer,H;Schulte,BA;Pasternack,G;Siegel,GJ;Spicer,SS
通讯作者：
Spicer,SS
两种类型的集合管富含线粒体（嵌入）的细胞：凝集素和带 3 细胞化学。
DOI：
10.1152/ajpcell.1986.251.3.c347
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Schuster,VL;Bonsib,SM;Jennings,ML
通讯作者：
Jennings,ML
影响因子：
56.9
作者：
LOHSE, MJ;BENOVIC, JL;LEFKOWITZ, RJ
通讯作者：
LEFKOWITZ, RJ
小鼠S抗原的结构分析。
影响因子：
3.5
作者：
Tsuda,M;Syed,M;Bugra,K;Whelan,JP;McGinnis,JF;Shinohara,T
通讯作者：
Shinohara,T
二氧化碳引起分离的灌注近端管和集合管中含有 H 泵的囊泡的胞吐作用。
DOI：
10.1172/jci111871
发表时间：
1985
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Schwartz,GJ;Al-Awqati,Q
通讯作者：
Al-Awqati,Q