Hair as a biomarker of environmental manganese exposure.
Hair as a biomarker of environmental manganese exposure.
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DOI：
10.1021/es3035297
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发表时间：
2013-02-05
影响因子：
11.4
通讯作者：
Smith, Donald R.
中科院分区：
文献类型：
--
作者：
Eastman, Rachel R.;Jursa, Tom P.;Benedetti, Chiara;Lucchini, Roberto G.;Smith, Donald R.
缺乏有效的生物标志物的锰（Mn）暴露在儿童仍然是一个主要的障碍，锰毒性的研究。我们开发了一种头发清洁方法，以确定头发作为Mn和其他金属（Pb，Cr，Cu）暴露生物标志物的效用，使用ICP-MS，扫描电子显微镜和激光消融ICP-MS来评估清洁效果。使用0.5%Triton X-100超声处理加1 N硝酸超声处理的清洁方法，有效地去除未经处理或故意被灰尘或锰污染的水污染的头发上的外源性金属污染。这种清洁方法，然后使用从儿童（n=121）的头发样本在一个正在进行的研究环境锰暴露和相关的健康影响。平均头发锰水平为0.121 μg/g（中位数= 0.073 μg/g，范围= 0.011 - 0.736 μg/g），比其他儿科锰研究中报告的水平低约4 - 70倍。与对照组相比，生活在活跃但非历史性铁合金工厂排放附近的儿童的头发锰水平也显著较高（P<0.001）。这些数据表明，外源性金属污染的头发可以有效地清除外源性金属污染，他们证实了使用头发锰水平作为生物标志物的环境锰暴露在儿童。
The absence of well-validated biomarkers of manganese (Mn) exposure in children remains a major obstacle for studies of Mn toxicity. We developed a hair cleaning methodology to establish the utility of hair as an exposure biomarker for Mn and other metals (Pb, Cr, Cu), using ICP-MS, scanning electron microscopy, and laser ablation ICP-MS to evaluate cleaning efficacy. Exogenous metal contamination on hair that was untreated or intentionally contaminated with dust or Mn-contaminated water was effectively removed using a cleaning method of 0.5% Triton X-100 sonication plus 1N nitric acid sonication. This cleaning method was then used on hair samples from children (n=121) in an ongoing study of environmental Mn exposure and related health effects. Mean hair Mn levels were 0.121 μg/g (median = 0.073 μg/g, range = 0.011 – 0.736 μg/g), which are ~4 to 70-fold lower than levels reported in other pediatric Mn studies. Hair Mn levels were also significantly higher in children living in the vicinity of active, but not historic, ferroalloy plant emissions compared to controls (P<0.001). These data show that exogenous metal contamination on hair can be effectively cleaned of exogenous metal contamination, and they substantiate the use of hair Mn levels as a biomarker of environmental Mn exposure in children.
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影响因子：
11.4
作者：
Finkelstein, M. E.;George, D.;Smith, D. R.
通讯作者：
Smith, D. R.
居住在铁 - 曼加纳合金生产厂附近的儿童中的高锰。
影响因子：
3.4
作者：
Menezes-Filho JA;Paes CR;Pontes AM;Moreira JC;Sarcinelli PN;Mergler D
通讯作者：
Mergler D
DOI：
10.1097/01.jom.0000116900.49159.03
发表时间：
2004-03-01
影响因子：
3.2
作者：
Li, GJJ;Zhang, LL;Zheng, W
通讯作者：
Zheng, W
影响因子：
8.3
作者：
Mergler, D;Baldwin, M
通讯作者：
Baldwin, M
影响因子：
8.3
作者：
Dietz, MC;Ihrig, A;Triebig, G
通讯作者：
Triebig, G