Lower maternal body condition during pregnancy affects skeletal muscle structure and glut-4 protein levels but not glucose tolerance in mature adult sheep.
Lower maternal body condition during pregnancy affects skeletal muscle structure and glut-4 protein levels but not glucose tolerance in mature adult sheep.
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DOI：
10.1177/1933719113477494
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Green LR
中科院分区：
文献类型：
--
作者：
Costello PM;Hollis LJ;Cripps RL;Bearpark N;Patel HP;Sayer AA;Cooper C;Hanson MA;Ozanne SE;Green LR
次优的母体营养和身体成分与成年后代的代谢性疾病风险有关。我们假设，由于骨骼肌结构和胰岛素信号传导机制的变化，先前在来自较低身体状况评分（BCS）的母羊的年轻成年绵羊后代中观察到的葡萄糖稳态的适度破坏会随着年龄的增长而恶化。在怀孕前和怀孕期间喂养母羊以达到较低（L，n=10）或较高（H，n=14）的BCS。在210±4周时，雄性后代的基线血糖、葡萄糖耐量和离体肌条的基础葡萄糖摄取相似。LBCS后代的股总肌纤维密度（HBCS，343±15; LBCS，294±14纤维/mm 2，p<0.05）和快肌纤维密度（HBCS，226±10; LBCS 194±10纤维/mm 2，p<0.05）、毛细血管与肌纤维比率（HBCS，1.5±0.1; LBCS 1.2±0.1毛细血管：肌纤维，p<0.05）较低。在LBCS后代中，Akt 1的股肌蛋白水平较低（HBCS的83±7%，p<0.05），总GLUT-4增加（HBCS的157±6%，p<0.001）。尽管LBCS后代中总肌纤维密度降低，但成熟成年期的葡萄糖耐量正常。然而，这种适应可能会导致代谢控制在一个过度丰富的产后营养环境的并发症。
Sub-optimal maternal nutrition and body composition are implicated in metabolic disease risk in adult offspring. We hypothesized that modest disruption of glucose homeostasis previously observed in young adult sheep offspring from ewes of a lower body condition score (BCS) would deteriorate with age, due to changes in skeletal muscle structure and insulin signalling mechanisms. Ewes were fed to achieve a lower (L, n=10) or higher (H, n=14) BCS before and during pregnancy. Baseline plasma glucose, glucose tolerance and basal glucose uptake into isolated muscle strips was similar in male offspring at 210±4 weeks. Vastus total myofibre density (HBCS, 343±15; LBCS, 294±14 fibres/mm2, p<0.05) and fast myofibre density (HBCS, 226±10; LBCS 194±10 fibres/mm2, p<0.05), capillary to myofibre ratio (HBCS, 1.5±0.1; LBCS 1.2±0.1 capillary:myofibre, p<0.05) were lower in LBCS offspring. Vastus protein levels of Akt1 were lower (83±7% of HBCS, p<0.05), and total GLUT-4 was increased (157±6% of HBCS, p<0.001) in LBCS offspring, Despite the reduction in total myofibre density in LBCS offspring, glucose tolerance was normal in mature adult life. However such adaptations may lead to complications in metabolic control in an overabundant postnatal nutrient environment.
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DOI：
10.1007/bf03340110
发表时间：
1996-02-01
期刊：
AGING-CLINICAL AND EXPERIMENTAL RESEARCH
影响因子：
--
作者：
Muller, DC;Elahi, D;Andres, R
通讯作者：
Andres, R
影响因子：
8.2
作者：
Ozanne, SE;Jensen, CB;Vaag, AA
通讯作者：
Vaag, AA
DOI：
10.1016/0300-9629(94)90124-4
发表时间：
1994-10-01
期刊：
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-PHYSIOLOGY
影响因子：
--
作者：
LANGLEY, SC;BROWNE, RF;JACKSON, AA
通讯作者：
JACKSON, AA
影响因子：
39.2
作者：
Mi, J;Law, C;Barker, D
通讯作者：
Barker, D
DOI：
10.1098/rspb.2004.3001
发表时间：
2005-04-07
影响因子：
4.7
作者：
Gluckman, PD;Hanson, MA;Bateson, P
通讯作者：
Bateson, P