Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses.
Separate urinary bladder and prostate neurons in the central nervous system of the rat: simultaneous labeling with two immunohistochemically distinguishable pseudorabies viruses.
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DOI：
10.1186/1471-2202-3-8
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发表时间：
2002-07-12
期刊：
影响因子：
2.4
通讯作者：
Vera PL
中科院分区：
文献类型：
--
作者：
Nadelhaft I;Miranda-Sousa AJ;Vera PL
本研究同时在大鼠膀胱和前列腺的同一组织切片中检测病毒标记神经元的中枢神经系统分布。将两种化学性质不同的伪狂犬病病毒株同时注射到雄性Sprague道利大鼠（约280 gm）中。一种病毒被注入膀胱，另一种注入前列腺。孵育间隔2.25、2.5、2.75、3和4天后，对脊髓和脑切片进行免疫组织化学处理，以检测单个切片内的细胞，这些细胞分别被每种病毒标记或被两种病毒标记。每种病毒株都标记了一个单独的神经元群体，一些神经元被两种病毒株标记。膀胱病毒标记的神经元主要分布于L 6-S1脊髓背侧灰质连合、中间外侧区和背角浅层。前列腺病毒标记的神经元主要分布在L1-L2脊髓背侧灰质连合和中间外侧区。L1-L2双标中间神经元主要位于中间外侧区。在L 6-S1，它们被分为背侧灰质连合和中间外侧区。支配膀胱的脊髓神经元与支配前列腺的脊髓神经元明显不同。这种差异也存在于大脑中。不同意以前的报告，没有直接的解剖学证据副交感神经支配的前列腺观察。
This work examines the central nervous system distribution of virus-labeled neurons from the rat urinary bladder and the prostate simultaneously within the same tissue sections. Two immunohistochemically distinct pseudorabies virus strains were simultaneously injected into male Sprague Dawley rats (~280 gm). One virus was injected into the bladder and the other into the prostate. After incubation intervals of 2.25, 2.5, 2.75, 3 and 4 days, sections from the spinal cord and brain were processed immunohistochemically to detect cells, within a single section, which were labeled separately by each virus or were labeled by both viruses. Each strain of virus labeled a separate population of neurons and some neurons were labeled by both strains. The majority of neurons labeled by virus from the urinary bladder were found in the L6-S1 spinal cord segments within the dorsal gray commissure, the intermediolateral area and the superficial dorsal horn. Neurons labeled by virus from the prostate were mainly found in the L1-L2 spinal cord segments in the dorsal gray commissure and the intermediolateral areas. Double-labeled interneurons in L1-L2 were mainly located in the intermediolateral area. In L6-S1 they were divided between the dorsal gray commissure and the intermediolateral area. Spinal neurons innervating the bladder are clearly separate and different from those innervating the prostate. This difference also persists in the brain. In disagreement with previous reports, no direct anatomical evidence of parasympathetic innervation of the prostate was observed.
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影响因子：
2.5
作者：
NADELHAFT, I;BOOTH, AM
通讯作者：
BOOTH, AM
影响因子：
2.9
作者：
LOEWY, AD;HAXHIU, MA
通讯作者：
HAXHIU, MA
影响因子：
2.9
作者：
Nadelhaft, I;Vera, PL
通讯作者：
Vera, PL
影响因子：
2.5
作者：
NADELHAFT, I;VERA, PL;MISELIS, RR
通讯作者：
MISELIS, RR
影响因子：
3.4
作者：
Zermann, DH;Ishigooka, M;Schmidt, RA
通讯作者：
Schmidt, RA