Assessing the impact of nitrogen supplementation in oats across multiple growth locations and years with targeted phenotyping and high-resolution metabolite profiling approaches.
Assessing the impact of nitrogen supplementation in oats across multiple growth locations and years with targeted phenotyping and high-resolution metabolite profiling approaches.
复制标题
通过有针对性的表型分析和高分辨率代谢物分析方法评估燕麦在多个生长地点和年份中氮补充的影响。
DOI：
10.1016/j.foodchem.2021.129585
复制
发表时间：
2021-09-01
期刊：
影响因子：
8.8
通讯作者：
Howarth C
中科院分区：
文献类型：
--
作者：
Allwood JW;Martinez-Martin P;Xu Y;Cowan A;Pont S;Griffiths I;Sungurtas J;Clarke S;Goodacre R;Marshall A;Stewart D;Howarth C
通过3次田间试验，分析了4个冬用燕麦品种对氮素的反应。发展了一种新的高分辨率方法来描述代谢物的变化。提高产量的条件并不一定会导致更高的营养价值。品种的选择与氮素水平的应用同样重要。燕麦(Avena sativa L.)是一种健康的食物，富含膳食纤维(如β-葡聚糖)、抗氧化剂、矿物质和维生素。了解品种和作物管理对营养质量的影响是重要的。从产量、谷物品质和代谢物(通过GC-MS和LC-MS评估)方面，研究了四个燕麦品种在多个地点和年份对氮素水平增加的反应。建立了一种新的高分辨率UHPLC-PDA-MS/MS方法，改善了代谢物的浓缩、拆分和鉴定。联合表型分析表明，添加氮素后，氨基酸水平增加，总蛋白和含氮脂肪水平增加，而有益于健康的杂草类化合物减少。尽管氮素添加显著提高了谷物产量和β-葡聚糖含量，支持提高农业指南中建议的总氮素水平，但燕麦品种的选择以及对健康有益的次生代谢物和与氮肥相关的环境负担的负面影响需要进一步考虑。
The response to nitrogen of 4 winter oat varieties in three field trials was analysed. A novel high-resolution method was developed to profile metabolite changes. Conditions that enhance yield do not necessarily result in higher nutritional value. Choice of variety is of equally high importance as the nitrogen levels applied. Oats (Avena sativa L.) are a healthy food, being high in dietary fibre (e.g. β-glucans), antioxidants, minerals, and vitamins. Understanding the effect of variety and crop management on nutritional quality is important. The response of four oat varieties to increased nitrogen levels was investigated across multiple locations and years with respect to yield, grain quality and metabolites (assessed via GC- and LC- MS). A novel high-resolution UHPLC-PDA-MS/MS method was developed, providing improved metabolite enrichment, resolution, and identification. The combined phenotyping approach revealed that, amino acid levels were increased by nitrogen supplementation, as were total protein and nitrogen containing lipid levels, whereas health-beneficial avenanthramides were decreased. Although nitrogen addition significantly increased grain yield and β-glucan content, supporting increasing the total nitrogen levels recommended within agricultural guidelines, oat varietal choice as well as negative impacts upon health beneficial secondary metabolites and the environmental burdens associated with nitrogen fertilisation, require further consideration.
登录
查看更多内容
DOI：
10.1093/bioinformatics/btr079
发表时间：
2011-04-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Brown M;Wedge DC;Goodacre R;Kell DB;Baker PN;Kenny LC;Mamas MA;Neyses L;Dunn WB
通讯作者：
Dunn WB
影响因子：
8.8
作者：
de Bruijn, Wouter J. C.;van Dinteren, Sarah;Vincken, Jean-Paul
通讯作者：
Vincken, Jean-Paul
影响因子：
5.8
作者：
Finnan, John;Burke, Brendan;Spink, John
通讯作者：
Spink, John
影响因子：
3.1
作者：
Ma, B. L.;Zheng, Z.;Yan, W.
通讯作者：
Yan, W.
影响因子：
3.8
作者：
SAASTAMOINEN, M;PLAAMI, S;KUMPULAINEN, J
通讯作者：
KUMPULAINEN, J