Conceptus-Endometrium Crosstalk During Maternal Recognition of Pregnancy in Cattle1-猫眼课题宝

母牛妊娠识别过程中孕体与子宫内膜的串扰1

DOI：

发表时间：

2012

期刊：

Biology of Reproduction

影响因子：

3.6

通讯作者：

中科院分区：

生物学2区

文献类型：

作者：

S. Mamo;J. Mehta;N. Forde;P. McGettigan;P. Lonergan

摘要孵化后胚泡的成功生长发育和妊娠的建立是有能力的胚胎和可接受的子宫环境相互作用的结果。我们使用RNA-Seq技术研究了第16天牛胚胎和妊娠子宫内膜组织的整体转录组谱，以确定在妊娠识别期间参与对话的基因。采用严格的筛选标准，共获得16个 018转录本和16个 262转录本，具有不同的组织特异性表达谱。在这些转录本中，2261和2505个转录本是胚胎和子宫内膜特异的。使用Cytoscape软件，共鉴定出133个与子宫内膜上相应受体相互作用的胚胎配体和121个与子宫内膜上相应受体相互作用的子宫内膜配体。共有87种配体被检测到，其中46种是胚胎特异性的，34种是子宫内膜特异性的。这项研究是第一批提供了在母体识别怀孕的关键窗口期间，与子宫内膜上的受体相互作用的胚胎中潜在分泌分子的全面清单之一。已鉴定的组织特异性基因可能作为研究妊娠识别的候选基因，它们或其下游产物可能代表潜在的妊娠早期标记。

ABSTRACT Successful growth and development of the posthatching blastocyst and pregnancy establishment are a result of the interaction between a competent embryo and a receptive uterine environment. We examined the global transcriptome profiles of the Day 16 bovine conceptus and pregnant endometrium tissues using RNA-Seq to identify genes that contribute to the dialogue during the period of pregnancy recognition. Using stringent filtering criterion, a total of 16 018 and 16 262 transcripts of conceptus and pregnant endometrium origin, respectively, were identified with distinct tissue-specific expression profiles. Of these, 2261 and 2505 transcripts were conceptus and endometrium specific. Using Cytoscape software, a total of 133 conceptus ligands that interact with corresponding receptors on the endometrium and 121 endometrium ligands that interact with corresponding receptors on the conceptus were identified. While 87 ligands were commonly detected, 46 were conceptus specific and 34 endometrium specific. This study is one of the first to provide a comprehensive list of potentially secreted molecules in the conceptus that interact with receptors on the endometrium and vice versa during the critical window of maternal recognition of pregnancy. The identified tissue-specific genes may serve as candidates to study pregnancy recognition and they or downstream products may represent potential early markers of pregnancy.