Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies.
Large-scale hematopoietic differentiation of human induced pluripotent stem cells provides granulocytes or macrophages for cell replacement therapies.
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DOI：
10.1016/j.stemcr.2015.01.005
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发表时间：
2015-02-10
影响因子：
5.9
通讯作者：
Moritz T
中科院分区：
文献类型：
--
作者：
Lachmann N;Ackermann M;Frenzel E;Liebhaber S;Brennig S;Happle C;Hoffmann D;Klimenkova O;Lüttge D;Buchegger T;Kühnel MP;Schambach A;Janciauskiene S;Figueiredo C;Hansen G;Skokowa J;Moritz T
白介素-3（IL-3）能够支持广泛的造血细胞类型的增殖，而粒细胞刺激因子（G-CSF）和巨噬细胞CSF（M-CSF）代表髓样区域的临界细胞因子这是在基于多能茎细胞的造血分化模型中对此进行了研究克隆祖细胞超过2个月，而IL-3/g-CSF指向CD45+CD11B+CD15+CD15+CD16+CD66B+各种分化阶段的粒细胞，以表明细胞因子迁移，呼吸道迁移的能力，爆发反应和中性粒细胞 - 细胞陷阱的形成，暴露于IL-3/M-CSF导致CD45+CD11b+CD14+CD14+CD163+CD68+单核细胞/巨噬细胞型细胞能够进行吞噬和细胞因子分泌。 IL-3/G-CSF或IL-3/M-CSF对人多能干细胞的髓样规范允许延长髓样细胞的延长和大规模产生，因此也适用于细胞粘液和疾病模型研究作为基因和细胞疗法的应用。
IL-3-/M-CSF，G-CSF或GM-CSF对人PSC的髓样规范
M-CSF或G-CSF的大规模和连续生成M2-Mφ或粒细胞
功能性IPSC衍生的巨噬细胞或粒细胞类似于体内衍生的细胞
在本文中，Moritz及其同事表明，IL-3与M-CSF/G-CSF或GM-CSF结合使用，能够指示人类多能干细胞用于使用IL-3/G-CSF或使用IL-3/G-CSF。 IL-3/M-CSF允许分别长时间和大规模的人类粒细胞或巨噬细胞产生与体内衍生的对应物相比，它们表现出相似的功能。
Interleukin-3 (IL-3) is capable of supporting the proliferation of a broad range of hematopoietic cell types, whereas granulocyte colony-stimulating factor (G-CSF) and macrophage CSF (M-CSF) represent critical cytokines in myeloid differentiation. When this was investigated in a pluripotent-stem-cell-based hematopoietic differentiation model, IL-3/G-CSF or IL-3/M-CSF exposure resulted in the continuous generation of myeloid cells from an intermediate myeloid-cell-forming complex containing CD34+ clonogenic progenitor cells for more than 2 months. Whereas IL-3/G-CSF directed differentiation toward CD45+CD11b+CD15+CD16+CD66b+ granulocytic cells of various differentiation stages up to a segmented morphology displaying the capacity of cytokine-directed migration, respiratory burst response, and neutrophil-extracellular-trap formation, exposure to IL-3/M-CSF resulted in CD45+CD11b+CD14+CD163+CD68+ monocyte/macrophage-type cells capable of phagocytosis and cytokine secretion. Hence, we show here that myeloid specification of human pluripotent stem cells by IL-3/G-CSF or IL-3/M-CSF allows for prolonged and large-scale production of myeloid cells, and thus is suited for cell-fate and disease-modeling studies as well as gene- and cell-therapy applications. Myeloid specification of human PSCs by IL-3-/M-CSF, G-CSF, or GM-CSF Large-scale and continuous generation of M2-MΦ or granulocytes by M-CSF or G-CSF Functional iPSC-derived macrophages or granulocytes similar to in-vivo-derived cells In this article, Moritz and colleagues show that IL-3 in combination with M-CSF/G-CSF or GM-CSF is able to instruct human pluripotent stem cells toward a myeloid lineage. The use of IL-3/G-CSF or IL-3/M-CSF allows for prolonged and large-scale production of human granulocytes or macrophages, respectively, that demonstrate similar functionalities compared with their in-vivo-derived counterparts.
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影响因子：
2.7
作者：
EVANS, WH;WILSON, SM;MAGE, MG
通讯作者：
MAGE, MG
影响因子：
56.9
作者：
CLARK, SC;KAMEN, R
通讯作者：
KAMEN, R
肺泡巨噬细胞从胎儿单核细胞中发展，这些单核细胞在生命的第一周通过GM-CSF分化为长寿命细胞。
DOI：
10.1084/jem.20131199
发表时间：
2013-09-23
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Guilliams M;De Kleer I;Henri S;Post S;Vanhoutte L;De Prijck S;Deswarte K;Malissen B;Hammad H;Lambrecht BN
通讯作者：
Lambrecht BN
影响因子：
21.3
作者：
通讯作者：
--
影响因子：
4.4
作者：
Lacey, Derek C.;Achuthan, Adrian;Hamilton, John A.
通讯作者：
Hamilton, John A.