Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation.
Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation.
复制标题
免疫共沉淀揭示了与家蚕核多角体病毒的潜在蛋白质相互作用
作者：
家蚕核型多角体病毒（BmNPV）是一种典型的杆状病毒，是家蚕的主要病原之一。我们在体外重组病毒，并将其用作细胞和蚕体免疫沉淀实验的诱饵，获得了可能与BmNPV相互作用的蛋白质数据库。本研究为BmNPV受体的筛选和对BmNPV复制起关键作用的蛋白质的研究提供了蛋白质数据参考。在分子水平上研究家蚕核型多角体病毒（BmNPV）的防治也是十分重要的。病毒-宿主相互作用对于病毒复制、毒力和致病性至关重要。家蚕核型多角体病毒（BmNPV）是一种典型的杆状病毒，是蚕业中最常见、最有害的病原之一。在此，我们使用免疫共沉淀来鉴定与BmNPV具有潜在相互作用的候选蛋白。首先，利用MultiBac杆状病毒多基因表达系统构建了重组BV病毒颗粒rBmBV-egfp-p64-3× flag-gp 64 sp。用重组BV病毒感染家蚕细胞和大灶蚕进行免疫共沉淀实验。LC-MS/MS分析表明，从家蚕细胞和家蚕样品中分别获得了845和1368个候选蛋白。进行生物信息学分析（Gene Ontology，KEGG Pathway）以选择具有显著富集的蛋白，用于进一步确认对BmNPV复制的影响。结果表明，SEC 61和PIC对BmNPV的复制有促进作用，而FABP 1对BmNPV的复制有抑制作用。总之，本研究揭示了BmNPV在宿主中入侵和增殖的潜在蛋白，并为BmNPV潜在受体蛋白的鉴定提供了平台。
The Bombyx mori nucleopolyhedrovirus (BmNPV) is a typical model baculovirus, representing one of the major pathogens of the silkworm. We reconstituted the virus in vitro and used it as a bait for immunoprecipitation experiments on cells and silkworm bodies, obtaining a database of proteins potentially interacting with BmNPV. This study provides a protein data reference for the screening of BmNPV receptors and the study of proteins that play a key role in the replication of BmNPV. It is also important to deal with the prevention of BmNPV in silkworms at the molecular level. Virus–host interactions are critical for virus replication, virulence, and pathogenicity. The Bombyx mori nucleopolyhedrovirus (BmNPV) is a typical model baculovirus, representing one of the most common and harmful pathogens in sericulture. Herein, we used co-immunoprecipitation to identify candidate proteins with potential interactions with BmNPV. First, a recombinant BV virus particle rBmBV-egfp-p64-3×flag-gp64sp was constructed using a MultiBac baculovirus multigene expression system. Co-immunoprecipitation experiments were then performed with the recombinant BV virus infected with BmN cells and Dazao silkworms. LC-MS/MS analysis revealed a total of 845 and 1368 candidate proteins were obtained from BmN cells and silkworm samples, respectively. Bioinformatics analysis (Gene Ontology, KEGG Pathway) was conducted for selection of proteins with significant enrichment for further confirmation of the effects on BmNPV replication. Overall, the results showed that SEC61 and PIC promoted the replication of BmNPV, while FABP1 inhibited the replication of BmNPV. In summary, this study reveals the potential proteins involved in BmNPV invasion and proliferation in the host and provides a platform for identifying the potential receptor proteins of BmNPV.
登录
查看更多内容
影响因子：
64.5
作者：
Clausen, Thomas Mandel;Sandoval, Daniel R.;Esko, Jeffrey D.
通讯作者：
Esko, Jeffrey D.
影响因子：
--
作者：
Slack J;Arif BM
通讯作者：
Arif BM
BmNPV感染后易感和抗性家蚕品系选择性剪接差异的全转录组分析
影响因子：
2.8
作者：
Li, Gang;Zhou, Kaiyue;Xu, Anying
通讯作者：
Xu, Anying
影响因子：
3.8
作者：
Gomi, S;Majima, K;Maeda, S
通讯作者：
Maeda, S
影响因子：
3.4
作者：
ACKERMANN, HW;SMIRNOFF, WA
通讯作者：
SMIRNOFF, WA