Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection
Development of a novel RSIVD-resistant strain of red sea bream (Pagrus major) by marker-assisted selection combined with DNA-based family selection
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通过标记辅助选择与基于 DNA 的家系选择相结合，开发了一种新的 RSIVD 抗性红海鲷（真鲷）品系
DOI：
10.1016/j.aquaculture.2019.03.039
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发表时间：
2019
期刊：
影响因子：
4.5
通讯作者：
Takagi Motohiro
中科院分区：
文献类型：
--
作者：
Sawayama Eitaro;Kitamura Shin-Ichi;Nakayama Kei;Ohta Kohei;Okamoto Hiroyuki;Ozaki Akiyuki;Takagi Motohiro
我们利用标记辅助选择与基于 DNA 的家族选择相结合，开发了一种新的真鲷品系，该品系对红真鲷虹彩病毒病 (RSIVD) 具有抗性，并评估了其 G2 在农场环境中的存活率。 G1 群体源自单个 RSIVD 抗性雄性 (G0)。使用微卫星进行 DNA 亲子分析，鉴定了抗性雄性的 G1 后代。使用与RSIVD抗性性状(Pma4_014)连锁的数量性状基因座进行标记辅助选择，并选择继承Pma4_014(+)抗性等位基因的个体。两个 G2 种群于 2016 年和 2017 年生产，并在夏季在水产养殖设施中饲养了大约三个月。 2016 年，同一农场还饲养了正常生产群体，并比较了存活率。 2016年和2017年产生的G2群体存活率分别为78.8%和83.5%。 2016年正常生产队列的存活率为63.8%。 G2中耐药等位基因的分离模式为1(+/-):2(+/-):1(−/−)，Pma4_014的基因型频率是使用田间试验前后从2016年和2017年G2群体收集的约200个个体计算的。根据引入和存活的鱼类数量估计每种基因型的数量。具有抗性等位基因 (+/+) 和 (+/-) 的个体在这两年中表现出超过 80% 的估计存活率，但不具有抗性等位基因 (−/−) 的个体在这两年中表现出较低的存活率 (50%)。这一结果强烈表明，抗性等位基因显着影响RSIVD抗性，新开发的G2种群可用于进一步繁殖抗RSIVD红鲷鱼种。
We developed a novel strain of red sea breamPagrus majorthat showed resistance to red sea bream iridoviral disease (RSIVD) using marker-assisted selection combined with DNA-based family selection and evaluated survival of its G2in a farm environment. The G1population was derived from a single RSIVD-resistant male (G0). The G1progeny of the resistant male were identified by DNA parentage analysis using microsatellites. Marker-assisted selection using a quantitative trait locus linked to the RSIVD-resistant trait (Pma4_014) was conducted, and individuals inheriting the resistant allele ofPma4_014(+) were selected. Two G2populations were produced in 2016 and 2017, and reared for approximately three months, during the summer, at an aquaculture facility. A normal production cohort was also reared at the same farm in 2016 and survival compared. Survival rates of the G2populations produced in 2016 and 2017 were 78.8% and 83.5%, respectively. The survival rate of the 2016 normal production cohort was 63.8%. The segregation pattern of the resistant allele showed 1 (+/+): 2 (+/−): 1 (−/−) in G2, and genotype frequency ofPma4_014was calculated using approximately 200 individuals collected from 2016 and 2017 G2populations before and after the field trial. Numbers of each genotype were estimated based on the number of introduced and surviving fish. Individuals with the resistant allele, (+/+) and (+/−), showed over 80% of the estimated survival rates, but individuals without the resistant allele (−/−) showed lower survival (50%) in both years. This result strongly suggests that the resistant allele dominantly affects RSIVD resistance, and the newly developed G2population is useful for further propagation of RSIVD-resistant red sea bream fingerlings.
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影响因子：
0.6
作者：
INOUYE, K;YAMANO, K;SORIMACHI, M
通讯作者：
SORIMACHI, M
影响因子：
4.4
作者：
Moen T;Baranski M;Sonesson AK;Kjøglum S
通讯作者：
Kjøglum S
使用基于 DNA 的谱系追踪评估红鲷主要亲鱼的 RSIVD 抗性特征：现场研究
影响因子：
0.6
作者：
持田和彦;羽野健志;隠塚俊満;吉田吾郎;持田和彦・羽野健志・隱塚俊満・伊藤克敏・吉田吾郎;持田和彦・隱塚俊満・羽野健志・伊藤克敏・吉田吾郎;木村 雄一;持田和彦・隠塚俊満・羽野健志・伊藤克敏・吉田吾郎;持田和彦・隠塚俊満・羽野健志・伊藤克敏・吉田吾郎;Eitaro Sawayama and Motohiro Takagi
通讯作者：
Eitaro Sawayama and Motohiro Takagi
影响因子：
4.5
作者：
Fuji, Kanako;Hasegawa, Osamu;Okamoto, Nobuaki
通讯作者：
Okamoto, Nobuaki
影响因子：
4.5
作者：
Fuji, Kanako;Kobayashi, Kazunobu;Okamoto, Nobuaki
通讯作者：
Okamoto, Nobuaki