Bio-Rad's Bio-Plex® suspension array system, xMAP technology overview.

Bio-Rad's Bio-Plex® suspension array system, xMAP technology overview.
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DOI:
10.3109/13813455.2012.705301
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发表时间:
2012-10
影响因子:
3
通讯作者:
Houser B
Houser B
中科院分区:
医学4区
文献类型:
--
作者:
Houser B

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Bio-Plex® 系统利用 xMAP 技术允许对多达 100 种不同的分析物进行多重分析。多重分析使研究人员能够同时观察分析物,与传统免疫分析方法相比,在更短的时间内从更少的样品量中提供更多信息。与 ELISA 类似,xMAP 利用抗体夹心进行检测,但与 ELISA 的不同之处在于捕获底物和检测方法。 Bio-Plex® 检测不是平面,而是利用可差异检测的珠子组作为捕获溶液中分析物的底物,并采用荧光方法进行检测。这些微珠组可识别分析物,而检测抗体则用于测量分析物的数量。使用可差异检测的珠子可以同时识别和定量同一样品中的许多分析物。
The Bio-Plex® system utilizes xMAP technology to permit the multiplexing of up to 100 different analytes. Multiplex analysis gives researchers the ability to look at analytes simultaneously providing more information from less sample volume in less time than traditional immunoassay methods. Similar to ELISA, xMAP utilizes an antibody sandwich for detection but differs from ELISA in capture substrate and detection method. Rather than a flat surface, Bio-Plex®assays make use of differentially detectable bead sets as a substrate capturing analytes in solution and employs fluorescent methods for detection. These bead sets identify the analytes and detection antibodies are used to measure the quantity of analyte. The use of differentially detectable beads enables the simultaneous identification and quantification of many analytes in the same sample.
DOI: 10.1016/0014-5793(71)80319-8
发表时间: 1971-01-01
期刊: FEBS LETTERS
影响因子: 3.5
作者:
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