High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing.
High-Resolution Patterned Cellular Constructs by Droplet-Based 3D Printing.
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DOI：
10.1038/s41598-017-06358-x
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发表时间：
2017-08-01
影响因子：
4.6
通讯作者：
Bayley H
中科院分区：
文献类型：
--
作者：
Graham AD;Olof SN;Burke MJ;Armstrong JPK;Mikhailova EA;Nicholson JG;Box SJ;Szele FG;Perriman AW;Bayley H
生物打印是一种用于制造活组织的新兴技术，其允许细胞以预定的三维（3D）结构排列。然而，到目前为止，生物打印构建体包含以高分辨率图案化的多种细胞类型的例子有限。在这里，我们提出了一种低成本的方法，该方法采用3D打印含有哺乳动物细胞的水滴，以在油中产生稳健的图案化构建体，并将其可重复地转移到培养基中。以组织相关密度（107个细胞mL-1）和1 nL的高液滴分辨率打印人胚肾（HEK）细胞和绵羊间充质干细胞（oMSC）。打印的高分辨率3D几何形状具有≤200 μm的特征;这些特征包括树枝状细胞结、对角平面结和骨软骨界面。打印的细胞显示出高活力（平均90%），并且打印的结构内的HEK细胞显示出在培养条件下增殖。值得注意的是，一项为期五周的组织工程研究表明，打印的oMSC可以分化成软骨细胞系，产生含有II型胶原的软骨样结构。
Bioprinting is an emerging technique for the fabrication of living tissues that allows cells to be arranged in predetermined three-dimensional (3D) architectures. However, to date, there are limited examples of bioprinted constructs containing multiple cell types patterned at high-resolution. Here we present a low-cost process that employs 3D printing of aqueous droplets containing mammalian cells to produce robust, patterned constructs in oil, which were reproducibly transferred to culture medium. Human embryonic kidney (HEK) cells and ovine mesenchymal stem cells (oMSCs) were printed at tissue-relevant densities (107 cells mL−1) and a high droplet resolution of 1 nL. High-resolution 3D geometries were printed with features of ≤200 μm; these included an arborised cell junction, a diagonal-plane junction and an osteochondral interface. The printed cells showed high viability (90% on average) and HEK cells within the printed structures were shown to proliferate under culture conditions. Significantly, a five-week tissue engineering study demonstrated that printed oMSCs could be differentiated down the chondrogenic lineage to generate cartilage-like structures containing type II collagen.
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影响因子：
--
作者：
Bayley H;Cronin B;Heron A;Holden MA;Hwang WL;Syeda R;Thompson J;Wallace M
通讯作者：
Wallace M
影响因子：
13.6
作者：
Hinton TJ;Jallerat Q;Palchesko RN;Park JH;Grodzicki MS;Shue HJ;Ramadan MH;Hudson AR;Feinberg AW
通讯作者：
Feinberg AW
影响因子：
9
作者：
Jakab K;Norotte C;Marga F;Murphy K;Vunjak-Novakovic G;Forgacs G
通讯作者：
Forgacs G
影响因子：
4.6
作者：
Horváth L;Umehara Y;Jud C;Blank F;Petri-Fink A;Rothen-Rutishauser B
通讯作者：
Rothen-Rutishauser B
影响因子：
46.9
作者：
Kang, Hyun-Wook;Lee, Sang Jin;Atala, Anthony
通讯作者：
Atala, Anthony