Suicidal chemotaxis in bacteria.
Suicidal chemotaxis in bacteria.
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DOI：
10.1038/s41467-022-35311-4
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发表时间：
2022-12-09
影响因子：
16.6
通讯作者：
Foster, Kevin R.
中科院分区：
文献类型：
--
作者：
Oliveira, Nuno M.;Wheeler, James H. R.;Deroy, Cyril;Booth, Sean C.;Walsh, Edmond J.;Durham, William M.;Foster, Kevin R.
细菌通常生活在表面相关的社区中，在那里可以出现抗生素和其他化合物的陡峭梯度。虽然许多细菌物种在表面上移动，但令人惊讶的是，我们对这种抗生素梯度如何影响细胞运动知之甚少。在这里，我们研究了机会致病菌铜绿假单胞菌在几种抗生素的稳定空间梯度中的行为，方法是在微流体装置中跟踪数千个细胞，因为它们形成生物膜。出乎意料的是，这些实验揭示了细菌使用基于纤毛的（“抽搐”）运动来导航抗生素。我们的分析表明，这种行为是由抗生素对细胞影响的一般反应驱动的。迁移细菌在数小时内达到比其最低抑制浓度高数百倍的抗生素浓度，并保持高度运动性。然而，分离细胞-使用流体壁微流体装置-揭示了这些细菌是终端，无法繁殖。尽管正在走向死亡，但迁移的细胞能够进入自杀程序，释放杀死其他细菌的细菌素。这种行为表明，这些细胞对抗生素的反应就像它们来自附近生长的竞争群体一样，诱导它们入侵和攻击。因此，临床抗生素有可能诱使细菌死亡。细菌通过趋化性对营养物质和其他化合物作出反应，但对它们对抗生素的反应知之甚少。通过跟踪抗生素梯度中的细胞，作者表明表面附着的铜绿假单胞菌以似乎是自杀性攻击策略的方式向抗生素移动。
Bacteria commonly live in surface-associated communities where steep gradients of antibiotics and other chemical compounds can occur. While many bacterial species move on surfaces, we know surprisingly little about how such antibiotic gradients affect cell motility. Here, we study the behaviour of the opportunistic pathogen Pseudomonas aeruginosa in stable spatial gradients of several antibiotics by tracking thousands of cells in microfluidic devices as they form biofilms. Unexpectedly, these experiments reveal that bacteria use pili-based (‘twitching’) motility to navigate towards antibiotics. Our analyses suggest that this behaviour is driven by a general response to the effects of antibiotics on cells. Migrating bacteria reach antibiotic concentrations hundreds of times higher than their minimum inhibitory concentration within hours and remain highly motile. However, isolating cells - using fluid-walled microfluidic devices - reveals that these bacteria are terminal and unable to reproduce. Despite moving towards their death, migrating cells are capable of entering a suicidal program to release bacteriocins that kill other bacteria. This behaviour suggests that the cells are responding to antibiotics as if they come from a competing colony growing nearby, inducing them to invade and attack. As a result, clinical antibiotics have the potential to lure bacteria to their death. Bacteria respond to nutrients and other compounds via chemotaxis, but little is known of their responses to antibiotics. By tracking cells in antibiotic gradients, the authors show that surface-attached Pseudomonas aeruginosa move towards antibiotics in what appears to be a suicidal attack strategy.
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DOI：
10.1073/pnas.1105073108
发表时间：
2011-08-02
影响因子：
11.1
作者：
Jin, Fan;Conrad, Jacinta C.;Wong, Gerard C. L.
通讯作者：
Wong, Gerard C. L.
影响因子：
3.2
作者：
ADLER, J;HAZELBAUER, GL;DAHL, MM
通讯作者：
DAHL, MM
影响因子：
64.5
作者：
Basler M;Ho BT;Mekalanos JJ
通讯作者：
Mekalanos JJ
影响因子：
7.7
作者：
Limoli, Dominique H.;Warren, Elizabeth A.;O'Toole, George A.
通讯作者：
O'Toole, George A.
影响因子：
9.5
作者：
Deroy, Cyril;Wheeler, James H. R.;Rumianek, Agata N.;Cook, Peter R.;Durham, William M.;Foster, Kevin;Walsh, Edmond J.
通讯作者：
Walsh, Edmond J.