Metagenomic and metabolomic analyses unveil dysbiosis of gut microbiota in chronic heart failure patients.
Metagenomic and metabolomic analyses unveil dysbiosis of gut microbiota in chronic heart failure patients.
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宏基因组学和代谢组学分析揭示了慢性心力衰竭患者肠道微生物群的失调
DOI：
10.1038/s41598-017-18756-2
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发表时间：
2018-01-12
影响因子：
4.6
通讯作者：
Cai J
中科院分区：
文献类型：
--
作者：
Cui X;Ye L;Li J;Jin L;Wang W;Li S;Bao M;Wu S;Li L;Geng B;Zhou X;Zhang J;Cai J
以前的研究表明，慢性心力衰竭（CHF）可能存在肠道微生物群失调。然而，缺乏直接证据。在这项研究中，我们调查了CHF患者肠道菌群的组成和代谢模式，为CHF患者肠道菌群失调提供直接证据和全面了解。我们招募了53名CHF患者和41名对照。然后进行粪便样品的宏基因组学分析和粪便和血浆样品的代谢组学分析。我们发现，CHF患者的肠道微生物群组成与对照组有显著差异。CHF患者肠道菌群的基本特征是普氏粪杆菌减少和活泼瘤胃球菌增加。我们还观察到CHF患者中参与保护性代谢产物（如丁酸盐）和有害代谢产物（如三甲胺N-氧化物）代谢的肠道微生物失衡。CHF患者的粪便和血浆样本的代谢特征也显著改变。此外，粪便和血浆代谢模式的改变与CHF中的肠道微生物群失调相关。综上所述，我们发现CHF与不同的肠道微生物群生态失调有关，并确定了CHF中特定的核心细菌不平衡，沿着某些代谢物和肠道微生物变化之间的相关性。
Previous studies suggested a possible gut microbiota dysbiosis in chronic heart failure (CHF). However, direct evidence was lacking. In this study, we investigated the composition and metabolic patterns of gut microbiota in CHF patients to provide direct evidence and comprehensive understanding of gut microbiota dysbiosis in CHF. We enrolled 53 CHF patients and 41 controls. Metagenomic analyses of faecal samples and metabolomic analyses of faecal and plasma samples were then performed. We found that the composition of gut microbiota in CHF was significantly different from controls. Faecalibacterium prausnitzii decrease and Ruminococcus gnavus increase were the essential characteristics in CHF patients’ gut microbiota. We also observed an imbalance of gut microbes involved in the metabolism of protective metabolites such as butyrate and harmful metabolites such as trimethylamine N-oxide in CHF patients. Metabolic features of both faecal and plasma samples from CHF patients also significantly changed. Moreover, alterations in faecal and plasma metabolic patterns correlated with gut microbiota dysbiosis in CHF. Taken together, we found that CHF was associated with distinct gut microbiota dysbiosis and pinpointed the specific core bacteria imbalance in CHF, along with correlations between changes in certain metabolites and gut microbes.
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影响因子：
49.6
作者：
Bui, Anh L.;Horwich, Tamara B.;Fonarow, Gregg C.
通讯作者：
Fonarow, Gregg C.
影响因子：
30.3
作者：
Kelly CJ;Zheng L;Campbell EL;Saeedi B;Scholz CC;Bayless AJ;Wilson KE;Glover LE;Kominsky DJ;Magnuson A;Weir TL;Ehrentraut SF;Pickel C;Kuhn KA;Lanis JM;Nguyen V;Taylor CT;Colgan SP
通讯作者：
Colgan SP
影响因子：
30.3
作者：
Backhed, Fredrik;Roswall, Josefine;Jun, Wang
通讯作者：
Jun, Wang
DOI：
10.1073/pnas.1215689109
发表时间：
2012-12-26
影响因子：
11.1
作者：
Craciun, Smaranda;Balskus, Emily P.
通讯作者：
Balskus, Emily P.
影响因子：
3.7
作者：
Kamo T;Akazawa H;Suda W;Saga-Kamo A;Shimizu Y;Yagi H;Liu Q;Nomura S;Naito AT;Takeda N;Harada M;Toko H;Kumagai H;Ikeda Y;Takimoto E;Suzuki JI;Honda K;Morita H;Hattori M;Komuro I
通讯作者：
Komuro I