Regulation of c-myb oncogene expression in immature and mature murine T cells.
Regulation of c-myb oncogene expression in immature and mature murine T cells.
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未成熟和成熟鼠 T 细胞中 c-myb 癌基因表达的调节。
DOI：
10.1016/0161-5890(93)90001-r
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发表时间：
1993
影响因子：
3.6
通讯作者：
Subler,MA
中科院分区：
文献类型：
--
作者：
Mountz,JD;Subler,MA
c-myboncogene编码一种核结合蛋白，该蛋白可能在早期T细胞发育的分化过程中发挥重要作用。然而，GC启动子位点中功能重要的转录区域尚未明确，该启动子位点在T细胞分化中的调控意义尚未确定。因此，启动子强度是通过测量EL-4细胞提取物中的CAT活性来确定的，这些细胞提取物被含有克隆的5 ' myb基因片段的CAT表达载体转染。逐步去除ATG起始密码子上游- 2300bp至- 346bp之间的DNA序列导致CAT活性逐渐丧失50%，而删除ATG起始密码子上游- 346至- 295和- 232至- 155bp之间的DNA序列可消除启动子活性。猫的分析活动各种细胞系转染后这些相同的结构,发现相同的两个启动子区域所需高猫活动所有的细胞系,包括小鼠细胞系表达α/β细胞和高水平的c-myb (BW5147),α/β细胞和低水平的c-myb (Yac-1),或γ/δTCR (KN 12.1和2.4 KN T)小鼠成纤维细胞T细胞系(NIH-3T3),和一个人类上皮细胞系(海拉)。然而，在小鼠细胞系中，CAT活性与c- myb基因的稳态表达水平无关。我们的数据表明，c-myboncogene启动子是组成性表达的，高度依赖于5'mybgene的有限区域，需要两个DNA元件才能达到最佳活性，并且在多种T细胞系中都起作用。
The c-myboncogene encodes a nuclear binding protein which may play a major role in differentiation during early T cell development. However, the functionally important transcription regions in the GC promoter site have not been defined and the significance of the regulation of this promoter site in T cell differentiation has not been determined. Therefore, the promoter strength was determined by measurement of the CAT activity in cell extracts of EL-4 cells that were transfected with a CAT expression vector that contained cloned segments of the 5′mybgene. Stepwise removal of DNA sequences between −2300bp and −346bp upstream from the ATG initiation codon resulted in a gradual loss of 50% of CAT activity, whereas deletion of DNA sequences from −346 to −295 and −232 to −155bp upstream from the ATG initiation codon eliminated promoter activity. On analysis of the CAT activity after transfection of various cell lines with these same constructs, it was found that the same two promoter regions were required for high CAT activity in all the cell lines, including murine cell lines which express the α /β TCR and high levels of c-myb(BW5147), the α/β TCR and low levels of c-myb(Yac-1), or the γ/δ TCR (KN 12.1 and KN 2.4 T), a murine fibroblast T cell line (NIH-3T3), and a human epithelial cell line (HeLa). However, the CAT activity did not correlate with steady state levels of expression of the c-mybgene in the murine cell lines. Our data indicate that the c-myboncogene promoter is constitutively expressed, is highly dependent on a limited region of the 5′mybgene, requires two DNA elements for optimal activity, and is functional in diverse T cell lines.
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影响因子：
2.9
作者：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
64.8
作者：
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通讯作者：
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