Calcitonin gene-related peptide excites myenteric neurons.
Calcitonin gene-related peptide excites myenteric neurons.
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降钙素基因相关肽兴奋肌间神经元。
DOI：
10.1016/0014-2999(86)90601-1
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发表时间：
1986
影响因子：
5
通讯作者：
Wood,JD
中科院分区：
文献类型：
--
作者：
Palmer,JM;Schemann,M;Tamura,K;Wood,JD
用细胞内方法记录离体豚鼠回肠肌间神经元的电行为。将降钙素基因相关肽（CGRP; 1 nM至1 gmM）和降钙素（1-100 μM）加入纵向肌-肌间神经丛制剂的灌流液中。两种肽也通过从细尖微量移液器的压力喷射来施加。CGRP，通过任一方法应用，引起了持久的去极化的细胞膜，这是剂量依赖性的（ED 50 =50 nM），并与输入电阻的增加，抑制后尖峰超极化电位和增强兴奋性，在所有的神经元进行了测试。增强的兴奋性反映了由细胞内注射的恒定电流去极化脉冲诱发的动作电位的数量显着增加。增强的兴奋性也是明显的，如在CGRP诱导的去极化的波峰处出现的一系列尖峰。CGRP的兴奋作用模拟缓慢的突触兴奋。应用降钙素并没有引起任何变化，肌间神经元的电行为。结果与CGRP在肠神经系统中的神经递质或神经调质作用一致，并表明它可能参与胃肠效应系统的局部神经体液调节。
Intracellular methods were used to record electrical behavior of myenteric neurons in guinea-pig ileum in vitro. Calcitonin gene-related peptide (CGRP; 1 nM to 1 gmM) and calcitonin (1–100 μM) were applied by addition to the superfusion solution of longitudinal muscle-myenteric plexus preparations. Both peptides were applied also by pressure ejection from fine-tipped micropipettes. CGRP, applied by either method, evoked a long-lasting depolarization of the cell membranes that was dose-dependent (ED50=50 nM) and was associated with an increase in the input resistance, suppression of post-spike hyperpolarizing potentials and enhanced excitability in all neurons that were tested. The enhanced excitability was reflected by a significant increase in the number of action potentials evoked by intracellular injection of constant current depolarizing pulses. Enhanced excitability also was apparent as a train of spikes that appeared at the crests of the CGRP-induced depolarization. The excitatory action of CGRP simulated slow synaptic excitation. Application of calcitonin did not evoke any changes in electrical behavior of myenteric neurons. The results are consistent with a neurotransmitter or neuromodulator role for CGRP in the enteric nervous system and suggest that it may participate in local neurohumoral regulation of gastrointestinal effector systems.
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DOI：
--
发表时间：
1980
期刊：
Journal of Physiology
影响因子：
--
作者：
S. Johnson;Y. Katayama;R. North
通讯作者：
R. North
影响因子：
5.5
作者：
HIRST, GDS;HOLMAN, ME;SPENCE, I
通讯作者：
SPENCE, I
豚鼠回肠肌间神经元中缓慢的钙依赖性钾电流。
DOI：
--
发表时间：
1985
期刊：
Journal of Physiology
影响因子：
--
作者：
G. Hirst;S. Johnson;D. V. van Helden
通讯作者：
D. V. van Helden
降钙素和降钙素基因相关肽在靶组织受体位点的相互作用。
影响因子：
56.9
作者：
D. Goltzman;J. Mitchell
通讯作者：
J. Mitchell
降钙素——控制大鼠肠道蠕动模式的中枢神经系统作用
影响因子：
3
作者：
L. Buéno;J. Fioramonti;J. Ferré
通讯作者：
J. Ferré