Effects of Iron Deficiency on Serum Metabolome, Hepatic Histology, and Function in Neonatal Piglets
Effects of Iron Deficiency on Serum Metabolome, Hepatic Histology, and Function in Neonatal Piglets
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缺铁对新生仔猪血清代谢组、肝组织学和功能的影响
DOI：
10.3390/ani10081353
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发表时间：
2020-08
期刊：
影响因子：
3
通讯作者：
Yulong Yin
中科院分区：
文献类型：
--
作者：
Zhenglin Dong;Dan Wan;Huansheng Yang;Guanya Li;Yiming Zhang;Xihong Zhou;Xin Wu;Yulong Yin
简单总结 缺铁是现代集约化养殖系统中哺乳期新生猪严重的营养缺乏症，会导致免疫反应受损、感染风险和生长迟缓。目的是通过超高效液相色谱-串联质谱仪 (UPLCMS/MS) 进行定量和定性分析，确定新生猪缺铁如何改变血清代谢组谱。目前的研究结果表明，缺铁导致新生仔猪肝脏酪氨酸代谢、苯丙氨酸代谢、胆汁分泌、初级胆汁酸生物合成、类固醇生物合成以及尿素循环酶活性上调等一系列代谢变化。摘要 很少有研究关注铁对新生仔猪代谢过程变化的影响。在本研究中，16 头新生仔猪被随机分配到两组。第一组仔猪肌肉注射 150 mg 右旋糖酐铁作为阳性对照 (CON)，第二组不补充铁作为缺铁阴性对照 (ID)。第8天，分析铁状态、血清生化参数、血清代谢组、肝组织学和肝脏代谢基因表达。结果表明，未补充铁的仔猪在第 8 天时铁值显着降低，血液尿素氮浓度升高 (p < 0.05)。血清代谢组分析显示，缺铁仔猪血清赖氨酸、亮氨酸、酪氨酸、蛋氨酸和胆固醇浓度显着降低，而3-甲基二氧吲哚、鹅去氧胆酸、吲哚乙酸、二十碳二烯酸、苯丙酮酸、泛酸、熊胆酸和胆酸浓度显着升高（p < 0.05）。此外，缺铁猪中 cyp7a1 和尿素循环酶（鸟氨酸转氨甲酰酶和精氨琥珀酸合成酶）的表达显着增加（p < 0.05）。目前的实验结果表明，未补充铁的新生仔猪在出生后8天内就会降至临界贫血。缺铁导致新生仔猪肝脏酪氨酸代谢、苯丙氨酸代谢、胆汁分泌、初级胆汁酸生物合成、类固醇生物合成以及尿素循环酶活性上调等一系列代谢变化，提示对新生仔猪代谢健康的早期影响。
Simple Summary Iron deficiency is a serious nutrient deficiency in neonatal pigs during the suckling period in modern intensive farming systems and leads to impaired immune response, infection risks, and retardation of growth. The objective was to determine how iron deficiency in neonatal pigs alters the serum metabolomic profile using quantitative and qualitative analysis by ultra-performance liquid chromatography-tandem mass spectrometer (UPLCMS/MS). The current results revealed that iron deficiency led to a series of metabolic changes involved in tyrosine metabolism, phenylalanine metabolism, bile secretion, primary bile acid biosynthesis, steroid biosynthesis, and upregulated activities of the urea cycle enzymes in the liver of neonatal piglets. Abstract Few studies focused on the effects of iron on characterizing alterations of metabolic processes in neonatal piglets. In the present study, 16 neonatal piglets were randomly assigned to two groups. In the first group piglets were given an intramuscularly injection of iron dextran at 150 mg as a positive control (CON) and the second group were not supplemented with iron as a negative control for iron deficiency (ID). At day 8, iron status, serum biochemical parameters, serum metabolome, hepatic histology, and hepatic expression of genes for the metabolism were analyzed. Results indicated that piglets without iron supplementation had significantly reduced iron values and increased blood urea nitrogen concentrations at day 8 (p < 0.05). Analysis of serum metabolome revealed that concentrations of serum lysine, leucine, tyrosine, methionine, and cholesterol were significantly decreased while concentrations of 3-Methyldioxyindole, chenodeoxycholate acid, indoleacetic acid, icosadienoic acid, phenylpyruvic acid, pantothenic acid, ursocholic acid, and cholic acid were significantly increased in iron deficient piglets (p < 0.05). Furthermore, expressions of cyp7a1 and the urea cycle enzyme (ornithinetranscarbamoylase and argininosuccinate synthetase) were significantly increased in iron deficient pigs (p < 0.05). The present experimental results indicated that neonatal piglets without iron supplementation drop to borderline anemia within 8 days after birth. Iron deficiency led to a series of metabolic changes involved in tyrosine metabolism, phenylalanine metabolism, bile secretion, primary bile acid biosynthesis, steroid biosynthesis, and upregulated activities of the urea cycle enzymes in the liver of neonatal piglets, suggesting early effects on metabolic health of neonatal piglets.
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影响因子：
0.8
作者：
Jong Weon Choi;Soon Ki Kim;S. Pai
通讯作者：
Jong Weon Choi;Soon Ki Kim;S. Pai
影响因子：
3.7
作者：
Radlowski EC;Conrad MS;Lezmi S;Dilger RN;Sutton B;Larsen R;Johnson RW
通讯作者：
Johnson RW
影响因子：
2.1
作者：
A. Egeli;T. Framstad;H. Morberg
通讯作者：
A. Egeli;T. Framstad;H. Morberg
DOI：
--
发表时间：
2012
期刊：
Journal of Veterinary Advances
影响因子：
--
作者：
Victor Ishaya
通讯作者：
Victor Ishaya
DOI：
--
发表时间：
2014-02
期刊：
Journal of Research in Medical Sciences : The Official Journal of Isfahan University of Medical Sciences
影响因子：
--
作者：
Nazanin Abbaspour;R. Hurrell;R. Kelishadi
通讯作者：
Nazanin Abbaspour;R. Hurrell;R. Kelishadi