A rat model of chronic postinflammatory visceral pain induced by deoxycholic acid.
A rat model of chronic postinflammatory visceral pain induced by deoxycholic acid.
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DOI：
10.1053/j.gastro.2008.08.051
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发表时间：
2008-12
期刊：
影响因子：
29.4
通讯作者：
Sun Y
中科院分区：
文献类型：
--
作者：
Traub RJ;Tang B;Ji Y;Pandya S;Yfantis H;Sun Y
慢性内脏痛觉过敏被认为是肠易激综合征（IBS）的重要病理生理学症状，既往胃肠道炎症是IBS发生的重要病因。虽然有几种动物模型的成年内脏高敏感性新生儿扰动或急性结肠炎症，有没有合适的模型炎症后慢性内脏痛觉过敏。本研究旨在建立大鼠结肠炎后慢性内脏痛觉过敏模型。每天将脱氧胆酸（DCA）滴注到大鼠结肠中，持续3天，并对动物进行长达4周的试验。DCA在3天内诱导轻度、一过性结肠炎症，并在3周内消退。一个夸张的内脏反应，涉及疼痛的机械刺激，增加脊髓Fos的表达，结肠传入和背角神经元的活动是明显的1周，并持续至少4周，表明慢性背角过度兴奋和内脏痛觉过敏。基于旷场行为，没有自发性疼痛。阿片受体活性显著增加。DCA诱导轻度、短暂性结肠炎，导致大鼠持续内脏痛觉过敏和牵涉痛，模拟炎症后IBS的某些方面。
Chronic visceral hyperalgesia is considered an important pathophysiological symptom in irritable bowel syndrome (IBS); previous gastrointestinal inflammation is a potent etiological factor for developing IBS. Although there are several animal models of adult visceral hypersensitivity following neonatal perturbation or acute colonic inflammation, there is no suitable model of post-inflammatory chronic visceral hyperalgesia. The aim of this study was to establish a model of chronic visceral hyperalgesia following colonic inflammation in the rat. Deoxycholic acid (DCA) was instilled into the rat colon daily for 3 days and animals were tested for up to 4 weeks. DCA induced mild, transient colonic inflammation within 3 days that resolved within 3 weeks. An exaggerated visceromotor response, referred pain to mechanical stimulation, increased spinal Fos expression, and colonic afferent and dorsal horn neuron activity were apparent by 1 week and persisted for at least 4 weeks, indicating chronic dorsal horn hyperexcitability and visceral hyperalgesia. There was no spontaneous pain, based on open field behavior. There was a significant increase in opioid receptor activity. DCA induces mild, transient colitis, resulting in persistent visceral hyperalgesia and referred pain in rats, modeling some aspects of post-inflammatory IBS.
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发表时间：
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影响因子：
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