Planctomycetes do possess a peptidoglycan cell wall.
Planctomycetes do possess a peptidoglycan cell wall.
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DOI：
10.1038/ncomms8116
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发表时间：
2015-05-12
影响因子：
16.6
通讯作者：
Jogler, Christian
中科院分区：
文献类型：
--
作者：
Jeske, Olga;Schueler, Margarete;Schumann, Peter;Schneider, Alexander;Boedeker, Christian;Jogler, Mareike;Bollschweiler, Daniel;Rohde, Manfred;Mayer, Christoph;Engelhardt, Harald;Spring, Stefan;Jogler, Christian
大多数细菌都含有肽聚糖 (PG) 细胞壁，这对于维持形状至关重要，对细胞分裂也很重要。相比之下，浮霉菌被认为可以产生不含 PG 的蛋白质细胞壁。 PG 的明显缺失已被用作所有细菌谱系假定的浮霉菌祖先的论据。在这里，我们采用多种生物信息学方法表明，浮霉菌基因组编码 PG 合成所需的蛋白质。此外，我们通过生化证明浮霉菌中存在 PG 的糖和肽成分。此外，光学和电子显微镜实验揭示了浮霉菌 PG 球囊对溶菌酶处理敏感。最后，冷冻电子断层扫描表明浮霉菌具有典型的 PG 细胞壁，因此它们的细胞结构与其他革兰氏阴性细菌更相似。我们的发现为特立独行的浮霉菌的细胞结构和细胞分裂提供了新的线索。 浮霉菌似乎与所有其他细菌的细胞组织不同，并且明显缺乏肽聚糖（PG）细胞壁。 Jeske 等人在这里。表明浮霉菌确实具有典型的 PG 细胞壁，并且它们的细胞结构类似于革兰氏阴性细菌。
Most bacteria contain a peptidoglycan (PG) cell wall, which is critical for maintenance of shape and important for cell division. In contrast, Planctomycetes have been proposed to produce a proteinaceous cell wall devoid of PG. The apparent absence of PG has been used as an argument for the putative planctomycetal ancestry of all bacterial lineages. Here we show, employing multiple bioinformatic methods, that planctomycetal genomes encode proteins required for PG synthesis. Furthermore, we biochemically demonstrate the presence of the sugar and the peptide components of PG in Planctomycetes. In addition, light and electron microscopic experiments reveal planctomycetal PG sacculi that are susceptible to lysozyme treatment. Finally, cryo-electron tomography demonstrates that Planctomycetes possess a typical PG cell wall and that their cellular architecture is thus more similar to that of other Gram-negative bacteria. Our findings shed new light on the cellular architecture and cell division of the maverick Planctomycetes. Planctomycetes appear to differ from all other bacteria in their cellular organization and their apparent lack of a peptidoglycan (PG) cell wall. Here Jeske et al. show that Planctomycetes do possess a typical PG cell wall and that their cellular architecture resembles that of Gram-negative bacteria.
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