Effects of Sleeve Gastrectomy on Fecal Gut Microbiota and Short-Chain Fatty Acid Content in a Rat Model of Polycystic Ovary Syndrome.
Effects of Sleeve Gastrectomy on Fecal Gut Microbiota and Short-Chain Fatty Acid Content in a Rat Model of Polycystic Ovary Syndrome.
复制标题
袖状胃切除术对多囊卵巢综合征大鼠模型粪便肠道微生物群和短链脂肪酸含量的影响
DOI：
10.3389/fendo.2021.747888
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发表时间：
2021
影响因子：
5.2
通讯作者：
Xiang G
中科院分区：
文献类型：
--
作者：
Lin W;Wen L;Wen J;Xiang G
目的袖状胃切除术（SG）是治疗多囊卵巢综合征（PCOS），特别是肥胖患者的一种手术方法。在此，我们探讨了SG对PCOS大鼠肠道菌群的影响，并研究了肠道植物群与SG治疗PCOS疗效之间的关系。方法采用脱氢表雄酮（DHEA）注射液和高脂饲料诱导PCOS大鼠模型。对PCOS大鼠进行SG治疗，观察SG对PCOS大鼠粪便和肠道菌群及短链脂肪酸（SCFA）含量的影响。此外，还评估了肠道微生物群、SCFA含量和高雄激素血症或PCOS的其他标志之间的关联。结果在DHEA诱导的PCOS大鼠模型中，SG使厚壁菌门的丰度减少，拟杆菌门的丰度增加。在属水平上，PCOS-SG组中拟杆菌属和布劳特氏菌属的丰度增加，瘤胃球菌属、梭菌属和Alistipes属的丰度明显减少。此外，粪便SCFAs的水平，特别是丁酸，下降后SG。SG显著改善PCOS相关症状，如高雄激素血症、卵巢功能紊乱和糖耐量受损。拟杆菌属和布劳特氏菌属与粪便SCFAs、促黄体激素、睾酮和炎症因子水平呈负相关，瘤胃球菌属、梭菌属和Alistipes属与粪便SCFAs、促黄体激素、睾酮和炎症因子水平呈正相关。结论SG对PCOS相关性生殖代谢紊乱的改善与其对机体微生物类群和SCFA含量的调节有关。我们的研究结果提供了一个新的角度在PCOS的微生物机制SG后。
Purpose Sleeve gastrectomy (SG) is a surgical intervention for polycystic ovary syndrome (PCOS), especially for patients with obesity. Here, we explored the effects of SG on the gut microbiota of rats with PCOS and investigated the association between the intestinal flora and efficacy of SG in PCOS. Methods Dehydroepiandrosterone (DHEA) injection was administered alone and in combination with a high-fat diet to induce PCOS in rats. SG was performed in rats with PCOS, and the effects of SG on the fecal and gut microbiota and the short-chain fatty acid (SCFA) content were observed. Furthermore, the association among gut microbiota, SCFA content and hyperandrogenism or other hallmarks of PCOS was evaluated. Results The abundance of Firmicutes reduced and that of Bacteroidetes increased in response to SG in the DHEA-induced PCOS rat model. At the genus level, the abundances of Bacteroides and Blautia increased and those of Ruminococcus, Clostridium, and Alistipes reduced distinctly in the PCOS-SG groups. Moreover, the levels of fecal SCFAs, especially butyric acid, reduced after SG. SG significantly ameliorated PCOS-related symptoms such as hyperandrogenism, disrupted ovary function, and impaired glucose tolerance. Bacteroides and Blautia exhibited a negative correlation and Ruminococcus, Clostridium, and Alistipes exhibited a positive correlation with the levels of fecal SCFAs, luteinizing hormone, testosterone, and inflammatory factors. Conclusions The amelioration of PCOS-related reproductive and metabolic disorders following SG was associated with the regulation of microbial taxa and SCFA content. Our findings provide a novel perspective on the microbial mechanisms in PCOS after SG.
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影响因子：
1.6
作者：
Macfarlane, George T.;Macfarlane, Sandra
通讯作者：
Macfarlane, Sandra
影响因子：
--
作者：
Damms-Machado A;Mitra S;Schollenberger AE;Kramer KM;Meile T;Königsrainer A;Huson DH;Bischoff SC
通讯作者：
Bischoff SC
影响因子：
24.5
作者：
Li, Zhuang;Yi, Chun-Xia;Wang, Yanan
通讯作者：
Wang, Yanan
影响因子：
64.8
作者：
Turnbaugh, Peter J.;Ley, Ruth E.;Gordon, Jeffrey I.
通讯作者：
Gordon, Jeffrey I.
影响因子：
64.5
作者：
De Vadder, Filipe;Kovatcheva-Datchary, Petia;Mithieux, Gilles
通讯作者：
Mithieux, Gilles