Transcellular transport of polymeric IgA in the rat hepatocyte: biochemical and morphological characterization of the transport pathway.
Transcellular transport of polymeric IgA in the rat hepatocyte: biochemical and morphological characterization of the transport pathway.
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大鼠肝细胞中聚合IgA的跨细胞运输：转运途径的生化和形态表征。
DOI：
10.1083/jcb.101.6.2113
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发表时间：
1985-12
影响因子：
7.8
通讯作者：
HUBBARD, AL
中科院分区：
文献类型：
--
作者：
HOPPE, CA;CONNOLLY, TP;HUBBARD, AL
多聚伊加（pIgA）由肝实质细胞（肝细胞）通过受体介导的过程从血液转运至胆汁。我们研究了TEPC 15小鼠骨髓瘤pIgA的细胞内途径。当从1微克到1毫克125 I-pIgA注射到大鼠的隐静脉，36%的运输作为完整的蛋白质进入胆汁中超过3小时的时间。胆汁中转运的125 I-pIgA浓度在注射后30-60 min达到最高，90 min时，最终转运的总125 I-pIgA中约有80%分泌到胆汁中。（125 I-pIgA-HRP）的转运程度和动力学与未结合的125 I-pIgA-HRP相似。pIgA，因此用于可视化转运途径。125 I-pIgA-HRP注射后2.5 ~ 10 min原位固定的肝脏的过氧化物酶细胞化学显示，标记结构从肝窦区向肝细胞质的中间和胆小管区进行性重新分布。虽然共轭含结构开始积累在胆小管区域在这些早期的时候，没有共轭物存在于胆汁中，直到20分钟。从7.5至45分钟注射后约30%的标记结构的区域，包含高尔基复合体和溶酶体，但是，我们没有发现任何证据表明，细胞器包含125 I-pIgA-HRP。肝细胞中至少85%的所有阳性结构为中位直径为110-160 nm的囊泡，其余结构为小管和多泡体。小泡在胆管区往往比那些在窦区大。连续切片显示，125 I-pIgA-HRP含结构相对简单（主要是囊泡），广泛的互连之间不存在的窦和胆小管区域的结构。
Polymeric IgA (pIgA) is transported by liver parenchymal cells (hepatocytes) from blood to bile via a receptor-mediated process. We have studied the intracellular pathway taken by a TEPC15 mouse myeloma pIgA. When from 1 microgram to 1 mg 125I-pIgA was injected into the saphenous vein of a rat, 36% was transported as intact protein into the bile over a 3-h period. The concentration of transported 125I-pIgA was maximal in bile 30-60 min after injection, and approximately 80% of the total 125I-pIgA ultimately transported had been secreted into bile by 90 min. A horseradish peroxidase-pIgA conjugate (125I-pIgA-HRP) was transported to a similar extent and with kinetics similar to that of unconjugated 125I-pIgA and was therefore used to visualize the transport pathway. Peroxidase cytochemistry of livers fixed in situ 2.5 to 10 min after 125I-pIgA-HRP injection demonstrated a progressive redistribution of labeled structures from the sinusoidal area to intermediate and bile canalicular regions of the hepatocyte cytoplasm. Although conjugate-containing structures began accumulating in the bile canalicular region at these early times, no conjugate was present in bile until 20 min. From 7.5 to 45 min after injection approximately 30% of the labeled structures were in regions that contained Golgi complexes and lysosomes; however, we found no evidence that either organelle contained 125I-pIgA-HRP. At least 85% of all positive structures in the hepatocyte were vesicles of 110-160-nm median diameters, with the remaining structures accounted for by tubules and multivesicular bodies. Vesicles in the bile canalicular region tended to be larger than those in the sinusoidal region. Serial sectioning showed that the 125I-pIgA-HRP-containing structures were relatively simple (predominantly vesicular) and that extensive interconnections did not exist between structures in the sinusoidal and bile canalicular regions.
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DOI：
10.1083/jcb.96.6.1548
发表时间：
1983-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Roman LM;Hubbard AL
通讯作者：
Hubbard AL
影响因子：
7.8
作者：
DUNN, WA;HUBBARD, AL
通讯作者：
HUBBARD, AL
影响因子：
56.9
作者：
RENSTON, RH;JONES, AL;HRADEK, GT
通讯作者：
HRADEK, GT
DOI：
10.1073/pnas.76.4.2008
发表时间：
1979-01-01
影响因子：
11.1
作者：
FISHER, MM;NAGY, B;UNDERDOWN, BJ
通讯作者：
UNDERDOWN, BJ
DOI：
10.1083/jcb.96.1.230
发表时间：
1983-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hubbard AL;Ma A
通讯作者：
Ma A